추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
28G9, monoclonal
종 반응성
mouse, rat, human
기술
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... CREB3L2(64764)
일반 설명
Cyclic AMP-responsive element-binding protein 3-like protein 2 (UniProt Q8BH52; also known as cAMP-responsive element-binding protein 3-like protein 2) is encoded by the Creb3l2 (also known as Bbf2h7, C530025K05Rik, SCIRR69) gene (Gene ID 208647) in murine species. Creb3l2/Bbf2h7 belongs to the old astrocyte specifically induced substance (OASIS) family of ER-resident transcription factors that function as ER stress transducers. OASIS members are ER transmembrane proteins that possess both a transcription-activation domain and a basic leucine zipper (bZIP) domain. Upon initial activation through regulated intramembrane proteolysis (RIP), OASIS family members are transported from the ER to the Golgi apparatus. Sequential cleavage by site-1 protease (S1P) and site-2 protease (S2P) releases the N-terminal fragment, which then translocates to the nucleus and activates the transcription of target genes. Under normal conditions, cellular BBF2H7 and OASIS levels are low due to degradation via the ubiquitin-proteasome pathway as a result of constant E3 ubiquitin ligase HRD1- (HMG-CoA reductase degradation 1-) mediated ubiquitination. ER stress is shown to disrupt their interaction with HRD1, resulting in enhanced stability of BBF2H7 and OASIS and an upregulation of their target genes.
면역원
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse BBF2H7/CREB3L2.
애플리케이션
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-BBF2H7/CREB3L2 Antibody, clone 28G9 is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of BBF2H7/CREB3L2.
Western Blotting Analysis: 0.25 µg/mL from a representative lot detected BBF2H7/CREB3L2 in 10 µg of mouse MC3T3-E1 preosteoblasts.
Immunoprecipitation: A representative lot co-immunoprecipitated ER-associated E3 Ub ligase Hrd1 with BBF2H7/CREB3L2 from rat C6 glioma cells only before, but not after, ER stress induction by thapsigargin treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
Western Blottting Analysis: A representative lot detected BBF2H7/CREB3L2 upregulation in murine ATDC5 chondrogenic cells after siRNA-mediated Hrd1 knockdown, as well as BBF2H7/CREB3L2 upregulation in HeLa cells upon proteasome inhibition or ER stress induction (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949.)
Immunocytochemistry: A representative lot detected enhanced ER & nuclear BBF2H7/CREB3L2 immunoreactivity in rat glioma C6 cells upon proteasome inhibitor MG132 treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
Immunoprecipitation: A representative lot co-immunoprecipitated ER-associated E3 Ub ligase Hrd1 with BBF2H7/CREB3L2 from rat C6 glioma cells only before, but not after, ER stress induction by thapsigargin treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
Western Blottting Analysis: A representative lot detected BBF2H7/CREB3L2 upregulation in murine ATDC5 chondrogenic cells after siRNA-mediated Hrd1 knockdown, as well as BBF2H7/CREB3L2 upregulation in HeLa cells upon proteasome inhibition or ER stress induction (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949.)
Immunocytochemistry: A representative lot detected enhanced ER & nuclear BBF2H7/CREB3L2 immunoreactivity in rat glioma C6 cells upon proteasome inhibitor MG132 treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
품질
Evaluated by Western Blotting in rat C6 glioma cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected BBF2H7/CREB3L2 in 10 µg of rat C6 glioma cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected BBF2H7/CREB3L2 in 10 µg of rat C6 glioma cell lysate.
표적 설명
~57-60 kDa observed. Uncharacterized band(s) may appear in some lysates.
물리적 형태
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
S Kondo et al.
Cell death and differentiation, 19(12), 1939-1949 (2012-06-19)
Endoplasmic reticulum (ER) stress transducers transduce signals from the ER to the cytoplasm and nucleus when unfolded proteins accumulate in the ER. BBF2 human homolog on chromosome 7 (BBF2H7) and old astrocyte specifically induced substance (OASIS), ER-resident transmembrane proteins, have
Daniele Pittari et al.
Frontiers in cell and developmental biology, 10, 986997-986997 (2022-11-01)
Upon progesterone stimulation, Endometrial Stromal Cells (EnSCs) undergo a differentiation program into secretory cells (decidualization) to release in abundance factors crucial for embryo implantation. We previously demonstrated that decidualization requires massive reshaping of the secretory pathway and, in particular, of
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