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Merck
모든 사진(2)

주요 문서

MABC1702

Sigma-Aldrich

Anti-PDGF-BB Antibody, clone 6A9.9.11

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

6A9.9.11, monoclonal

분자량

calculated mol wt 27.28 kDa
observed mol wt ~12 kDa

정제법

using protein G

종 반응성

human

포장

antibody small pack of 100 μg

기술

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

동형

IgG1κ

에피토프 서열

Internal

단백질 ID 수납 번호

UniProt 수납 번호

배송 상태

2-8°C

타겟 번역 후 변형

unmodified

일반 설명

Platelet-derived growth factor subunit B (UniProt: P01127; also known as PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, Becaplermin) is encoded by the PDGFB (also known as PDGF2, SIS) gene (Gene ID: 5155) in human. PDGFs are a family of four cystine-knot-type growth factors (PDGF-A, -B, -C and -D) that are dimeric mitogenic signaling molecules. In the early developmental stage, they drive the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages they participate in tissue remodeling, cellular differentiation, and morphogenesis. PDGF A and B can exist as two A subunits (PDGF-AA), two B subunits (PDGF-BB), or one of each (PDGF-AB). These dimeric forms are differentially expressed in various cell types. Their action is mediated via two types of receptors PDGFR and PDGFRβ, which belong to the class III receptor tyrosine kinases and exhibit different expression patterns and physiological roles. PDGFR signaling controls gastrulation and the development of several organs such as lung, intestine, skin, testis, kidney, bones, and neuroprotective tissues and PDGFRβ signaling is an essential regulator of early hematopoiesis and blood vessel formation. PDGF-BB is synthesized with a signal peptide (aa 1-20) and two propeptides (aa 21-81 and 191-241), which are cleaved off to generate the active form (aa 82-190). PDGF-BB is expressed at high levels in the heart, placenta, fetal kidney, and substantia nigra in the brain and at moderate levels in hippocampus, skeletal muscle, kidney, and lung. It is required for normal blood vessel development, and for normal development of kidney glomeruli and is shown to plays an important role in wound healing. It is released by platelets upon wounding. Mutations in PDGFB gene have been linked to idiopathic basal ganglia calcification that leads to a wide spectrum of neuropsychiatric symptoms, including Parkinsonism, dystonia tremor, ataxia, and dementia. (Ref.: Chen, PH., et al. (2013). Biochim. Biophys. Acta. 1834(10); 2176-2186).

특이성

Clone 6A9.9.11 is a mouse monoclonal antibody that detects Platelet-derived growth factor subunit B. It targets an epitope within the internal region.

면역원

Recombinant mature form (aa 82-190) of human Platelet-derived growth factor subunit B (PDGF-BB).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting with recombinant human PDGF-BB.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant PDGF-BB.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected PDGF-BB in human placenta tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PDGF-BB, clone 6A9.9.11, Cat. No. MABC1702, is a mouse monoclonal antibody that detects PDGF-BB and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

저장 및 안정성

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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