추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
6A9.9.11, monoclonal
분자량
calculated mol wt 27.28 kDa
observed mol wt ~12 kDa
정제법
using protein G
종 반응성
human
포장
antibody small pack of 100 μg
기술
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG1κ
에피토프 서열
Internal
단백질 ID 수납 번호
UniProt 수납 번호
배송 상태
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
Platelet-derived growth factor subunit B (UniProt: P01127; also known as PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, Becaplermin) is encoded by the PDGFB (also known as PDGF2, SIS) gene (Gene ID: 5155) in human. PDGFs are a family of four cystine-knot-type growth factors (PDGF-A, -B, -C and -D) that are dimeric mitogenic signaling molecules. In the early developmental stage, they drive the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages they participate in tissue remodeling, cellular differentiation, and morphogenesis. PDGF A and B can exist as two A subunits (PDGF-AA), two B subunits (PDGF-BB), or one of each (PDGF-AB). These dimeric forms are differentially expressed in various cell types. Their action is mediated via two types of receptors PDGFR and PDGFRβ, which belong to the class III receptor tyrosine kinases and exhibit different expression patterns and physiological roles. PDGFR signaling controls gastrulation and the development of several organs such as lung, intestine, skin, testis, kidney, bones, and neuroprotective tissues and PDGFRβ signaling is an essential regulator of early hematopoiesis and blood vessel formation. PDGF-BB is synthesized with a signal peptide (aa 1-20) and two propeptides (aa 21-81 and 191-241), which are cleaved off to generate the active form (aa 82-190). PDGF-BB is expressed at high levels in the heart, placenta, fetal kidney, and substantia nigra in the brain and at moderate levels in hippocampus, skeletal muscle, kidney, and lung. It is required for normal blood vessel development, and for normal development of kidney glomeruli and is shown to plays an important role in wound healing. It is released by platelets upon wounding. Mutations in PDGFB gene have been linked to idiopathic basal ganglia calcification that leads to a wide spectrum of neuropsychiatric symptoms, including Parkinsonism, dystonia tremor, ataxia, and dementia. (Ref.: Chen, PH., et al. (2013). Biochim. Biophys. Acta. 1834(10); 2176-2186).
특이성
Clone 6A9.9.11 is a mouse monoclonal antibody that detects Platelet-derived growth factor subunit B. It targets an epitope within the internal region.
면역원
Recombinant mature form (aa 82-190) of human Platelet-derived growth factor subunit B (PDGF-BB).
애플리케이션
Quality Control Testing
Evaluated by Western Blotting with recombinant human PDGF-BB.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant PDGF-BB.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected PDGF-BB in human placenta tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with recombinant human PDGF-BB.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant PDGF-BB.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected PDGF-BB in human placenta tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PDGF-BB, clone 6A9.9.11, Cat. No. MABC1702, is a mouse monoclonal antibody that detects PDGF-BB and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
물리적 형태
Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
저장 및 안정성
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
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