생물학적 소스
rat
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
3C1, monoclonal
종 반응성
human
기술
dot blot: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
human ... MNDA(4332)
관련 카테고리
일반 설명
Myeloid cell nuclear differentiation antigen (UniProt P41218) is encoded by the MNDA gene (Gene ID 4332) in human. MNDA is a member of the interferon-inducible p200 (IFI-200) family of proteins with a conserved HIN-200 domain (human MNDA a.a. 196-394) that mediate interaction with proteins and DNA. IFI-200 members possess antimicrobial, cell growth regulatory, differential regulatory and immunomodulatory properties. In bone marrow-derived- and mature neutrophils, MNDA is predominantly located in the nucleus. MNDA is cleaved by caspases in neutrophils undergoing apoptosis and relocated to the cytoplasm. MNDA is downregulated in myelodysplastic syndrome and completely absent in a mouse strain susceptible to B-cell plasmacytoma, while being markedly (1,000-fold) upregulated in a mouse strain resistant to B-cell plasmacytoma. Originally thought to be restricted to, myeloid cells, MNDA and other IFI-200 family members are also found to be expressed in a variety of non-myeloid cell types. MNDA is highly expressed in normal bone tissue, while being at markedly low levels in osteosarcoma cells. MNDA overexpression is shown to induce apoptosis, inhibit proliferation, and reduce the migration of osteosarcoma cells.
특이성
Clone 3C1 reacted with both denatured and non-denatured MNDA. Clone 3C1 reacted with nuclear extract from NMDA-expressing HL-60, but not non-MNDA-expressing BII, human myeloid leukemia cells (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).
면역원
Human peripheral blood granulocyte nuclei.
애플리케이션
Detect MNDA using this rat monoclonal Anti-MNDA Antibody, clone 3C1, Cat. No. MABC1170, validated for use in Dot Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Dot Blot Analysis: Clone 3C1 hybridoma culture supernatant reacted with nuclear extract from HL-60, but not BII, human myeloid leukemia cells (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).
Flow Cytometry Analysis: A representative lot (pre-conjugated with Alexa Fluor™ 488) detected MNDA expression among the granulocyte-macrophage (G-M) progenitors, but not among the nucleated red blood cells (nRBCs) and lymphoid cells in control and myelodysplastic syndromes (MDS) human marrow aspirates. A G-M progenitor cell population with downregulated MNDA expression was detected in most marrow samples from MDS cases and some MDS cases contain MNDA-negative G-M progenitor cells (Briggs, R.C., et al. (2006). Cancer Res. 66(9):4645-4651).
Immunocytochemistry Analysis: A representative lot detected MNDA nuclear immunoreactivity among HL-60 cells and NMDA-transfected, but not untransfected K562 cells. MNDA-negative HL-60 cells increased with time in cultures maintained without fresh medium for an extended period of time. MNDA translocated from nucleus to cytoplasm beginning at 3 hours and completed 4 hours following H2O2 addition to HL-60 cells (Briggs, R.C., et al. (2006). Cancer Res. 66(9):4645-4651).
Immunoprecipitation Analysis: A representative lot immunoprecipitated MNDA from HL-60 nuclear extract (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).
Western Blotting Analysis: A representative lot detected a time-dependent loss of MNDA in HL-60 cell lysate beginning at 4 hours following H2O2 treatment (Briggs, R.C., et al. (2006). Cancer Res. 66(9):4645-4651).
Western Blotting Analysis: Clone 3C1 hybridoma culture supernatant detected MNDA in HL-60 nuclear extract (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).
Flow Cytometry Analysis: A representative lot (pre-conjugated with Alexa Fluor™ 488) detected MNDA expression among the granulocyte-macrophage (G-M) progenitors, but not among the nucleated red blood cells (nRBCs) and lymphoid cells in control and myelodysplastic syndromes (MDS) human marrow aspirates. A G-M progenitor cell population with downregulated MNDA expression was detected in most marrow samples from MDS cases and some MDS cases contain MNDA-negative G-M progenitor cells (Briggs, R.C., et al. (2006). Cancer Res. 66(9):4645-4651).
Immunocytochemistry Analysis: A representative lot detected MNDA nuclear immunoreactivity among HL-60 cells and NMDA-transfected, but not untransfected K562 cells. MNDA-negative HL-60 cells increased with time in cultures maintained without fresh medium for an extended period of time. MNDA translocated from nucleus to cytoplasm beginning at 3 hours and completed 4 hours following H2O2 addition to HL-60 cells (Briggs, R.C., et al. (2006). Cancer Res. 66(9):4645-4651).
Immunoprecipitation Analysis: A representative lot immunoprecipitated MNDA from HL-60 nuclear extract (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).
Western Blotting Analysis: A representative lot detected a time-dependent loss of MNDA in HL-60 cell lysate beginning at 4 hours following H2O2 treatment (Briggs, R.C., et al. (2006). Cancer Res. 66(9):4645-4651).
Western Blotting Analysis: Clone 3C1 hybridoma culture supernatant detected MNDA in HL-60 nuclear extract (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
품질
Evaluated by Immunocytochemistry in HL-60 cells.
Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected MNDA in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized HL-60 cells.
Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected MNDA in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized HL-60 cells.
표적 설명
45.84 kDa calculated. ~55 kDa reported (Hudson, C.R., et al. (1988). Hybridoma. 7(6):541-553). Uncharacterized bands may be observed in some lysate(s).
물리적 형태
Format: Purified
Protein G purified.
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
기타 정보
Concentration: Please refer to lot specific datasheet.
법적 정보
ALEXA FLUOR is a trademark of Life Technologies
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
시험 성적서(COA)
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