추천 제품
product name
Anti-Neurofilament 200 kDa Antibody, clone RT97, clone RT97, Chemicon®, from mouse
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
RT97, monoclonal
종 반응성
mouse, human
종 반응성(상동성에 의해 예측)
rat
제조업체/상표
Chemicon®
기술
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)
일반 설명
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the Millipore Technical Support section.
특이성
The antibody recognizes an epitope present on human neurofilaments 200 kDa and both bovine 160 kDa and 200 kDa neurofilaments (2). lt reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type).
면역원
Rat brain protein preparation
애플리케이션
Anti-Neurofilament 200 kDa Antibody, clone RT97 is an antibody against Neurofilament 200 kDa for use in IH, IH(P) & WB.
Immunohistochemistry:
1-10 μg/mL
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Cerebellum
Optimal working dilutions must be determined by end user.
1-10 μg/mL
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Cerebellum
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
품질
Evaluated by Western Blot on Mouse brain membrane lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates.
표적 설명
200 kDa
물리적 형태
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8ºC from date of receipt.
분석 메모
Control
Brain
Cultured neurons
Brain
Cultured neurons
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A comparative study of a silver stain and monoclonal antibody reactions on Alzheimer's neurofibrillary tangles.
Journal of Neurology, Neurosurgery, and Psychiatry, 50, 1221-1224 (1987)
One year survival and significant reversal of motor deficits in parkinsonian rats transplanted with hESC derived dopaminergic neurons.
Biochemical and biophysical research communications null
Netrin-1 is a novel myelin-associated inhibitor to axon growth.
The Journal of Neuroscience null
Normalization of striatal tyrosine hydroxylase and reversal of motor impairment in experimental parkinsonism with intravenous nonviral gene therapy and a brain-specific promoter.
Human Gene Therapy null
Alzheimer's disease: immunoreactivity of neurofibrillary tangles with anti-neurofilament and anti-paired helical filament antibodies.
Brain Research, 310, 249-260 (1984)
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