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Merck
모든 사진(2)

Key Documents

MAB5254

Sigma-Aldrich

Anti-Neurofilament 160 kDa Antibody, clone NN18

clone NN18, Chemicon®, from mouse

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

NN18, monoclonal

종 반응성

guinea pig, chicken, rat, mouse, pig

종 반응성(상동성에 의해 예측)

human

제조업체/상표

Chemicon®

기술

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

동형

IgG1

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

chicken ... Nefm(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)

일반 설명

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

특이성

The antibody reacts with neurofilament 160 kD (Debus, E., 1982; Shaw, G., 1984).

면역원

Purified neurofilament polypeptides.

애플리케이션

Anti-Neurofilament 160 kDa Antibody, clone NN18 is an antibody against Neurofilament 160 kDa for use in IH, IH(P) & WB.
Immunohistochemistry:
5-10 µg/mL on frozen or paraffin embedded tissue. See protocols for details.

Western Blot:
1:1000. 7.5%-10% SDS-PAGE

Optimal working dilutions and protocols must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

품질

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.

표적 설명

160 kDa

결합

Replaces: CBL214

물리적 형태

Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

저장 및 안정성

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Do not freeze.

분석 메모

Control
Brain

Cultured neurons

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Lori Blanchfield et al.
Journal of immunology (Baltimore, Md. : 1950), 199(8), 2680-2691 (2017-09-10)
Of interest to the etiology of demyelinating autoimmune disease is the potential to aberrantly activate CD4+ T cells due to cross-recognition of multiple self-epitopes such as has been suggested for myelin oligodendrocyte glycoprotein epitope 35-55 (MOG35-55) and neurofilament medium protein
Ablation of proliferating cells in the CNS exacerbates motor neuron disease caused by mutant superoxide dismutase.
Audet, JN; Gowing, G; Paradis, R; Soucy, G; Julien, JP
Testing null
Ablation of proliferating microglia does not affect motor neuron degeneration in amyotrophic lateral sclerosis caused by mutant superoxide dismutase.
Gowing, G; Philips, T; Van Wijmeersch, B; Audet, JN; Dewil, M; Van Den Bosch, L; Billiau et al.
The Journal of Neuroscience null
Extended secondhand tobacco smoke exposure induces plasticity in nucleus tractus solitarius second-order lung afferent neurons in young guinea pigs.
Shin-Ichi Sekizawa,Chao-Yin Chen,Andrea G Bechtold,Jocelyn M Tabor,John M Bric et al.
The European Journal of Neuroscience null
Chuang-Yu Lin et al.
JCI insight, 4(18) (2019-09-20)
The control of voluntary skeletal muscle contraction relies on action potentials, which send signals from the motor neuron through the neuromuscular junction (NMJ). Although dysfunction of the NMJ causes various neuromuscular diseases, a reliable in vitro system for disease modeling

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