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Key Documents

MAB4625

Sigma-Aldrich

Anti-Bcl-X Antibody, clone 2H12

clone 2H12, Chemicon®, from mouse

동의어(들):

Bcl-2-like Protein

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

2H12, monoclonal

종 반응성

rat, pig, mouse, human

제조업체/상표

Chemicon®

기술

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG2a

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... BCL2L1(598)

일반 설명

Bcl-X has two isoforms, Bcl-XL (long), a 241-aa protein, and Bcl-XS (short), a 178-aa protein lacking a 63 aa domain that is well conserved among members of the Bcl-2 family.

특이성

MAB4625 recognizes a protein of 27 kDa, identified as the Bcl-X protein. Reacts with both Bcl-XS and Bcl-XL.

면역원

Synthetic peptide, aa 3-14 of human Bcl-X protein. This amino acid sequence is shared by human and murine Bcl-X protein.

애플리케이션

Research Category
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
This Anti-Bcl-X Antibody, clone 2H12 is validated for use in FC, IF, IP, WB, IH for the detection of Bcl-X.
Wstern Blot: 1μg/mL (Hsu, 1997a; Hsu, 1997b)

Flow Cytometry

Immunofluorescence Immunoprecipitation: native protein (2ug/mg protein lysate).

Immunohistochemistry (frozen and paraffin): 2-4ug/mL for 30 minutes at room temperature.

Optimal working dilutions must be determined by end user.

물리적 형태

Format: Purified
Protein A purified immunoglobulin in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

저장 및 안정성

Maintain at 2-8°C for up to 12 months.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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