MAB4419

Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2

clone 7F9.2, from mouse

• 동의어(들): Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 7F9.2, monoclonal
• 종 반응성: human, mouse
• 기술: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• 입력: sample type: human embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type: mouse embryonic stem cell(s)
• 동형: IgG1κ
• NCBI 수납 번호: NP_976034
• UniProt 수납 번호: P20263
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... POU5F1(5460) ; mouse ... Pou5F1(18999)

설명

일반 설명

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

특이성

Reactivity to other species has not been determined.

This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform.

면역원

Recombinant human Oct-4 protein, a.a. 1-145.

애플리케이션

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1].

Western Blotting: Recommended working dilution is 1 - 2 µg/mL.

Flow Cytometry: Recommended working dilution is 2 µg/mL.

Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200.

표적 설명

~39 kDa

결합

Replaces: MAB4305

물리적 형태

Format: Purified

Protein G sepharose column

Purified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3.

저장 및 안정성

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable