추천 제품
제품명
Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1, culture supernatant, clone 1, Chemicon®
생물학적 소스
mouse
Quality Level
항체 형태
culture supernatant
항체 생산 유형
primary antibodies
클론
1, monoclonal
종 반응성
bovine, rat, rabbit (weakly), human
반응하면 안 됨
guinea pig
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
western blot: suitable
동형
IgG2a
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... MBP(4155)
rat ... Mbp(24547)
일반 설명
The classic group of MBP isoforms (isoforms 4-14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Isoform 1 Golli-MBP1, HOG7, 33 kDa
Isoform 2 Golli-MBP2, HOG5, 21.5 kDa
Isoform 3 MBP1, 21.5 kDa
Isoform 4 MBP2, 20.2 kDa
Isoform 5 MBP3, 18.5 kDa
Isoform 6 MBP4, 17.2 kDa
(SP_P02686)
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Isoform 1 Golli-MBP1, HOG7, 33 kDa
Isoform 2 Golli-MBP2, HOG5, 21.5 kDa
Isoform 3 MBP1, 21.5 kDa
Isoform 4 MBP2, 20.2 kDa
Isoform 5 MBP3, 18.5 kDa
Isoform 6 MBP4, 17.2 kDa
(SP_P02686)
특이성
Reacts with MBP from human, bovine and rat, epitope 129-138
면역원
Bovine myelin basic protein
Epitope: a.a. 129-138
애플리케이션
Immunohistochemistry(paraffin) Analysis:
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistology on frozen sections at 1:10
Western Blot Analysis:
A previous lot of this antibody was used in Western Blot.
ELISA:
A 1:200-1:1,000 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in Radioimmunoassay.
Optimal working dilutions must be determined by end user.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistology on frozen sections at 1:10
Western Blot Analysis:
A previous lot of this antibody was used in Western Blot.
ELISA:
A 1:200-1:1,000 dilution of a previous lot was used in ELISA.
RIA:
A previous lot of this antibody was used in Radioimmunoassay.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
This Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1 is validated for use in ELISA, IH, IH(P), RIA, WB for the detection of Myelin Basic Protein.
품질
Routinely evaluated by immunohistochemistry on brain tissue.
Immunohistochemistry(paraffin) Analysis:
MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistochemistry(paraffin) Analysis:
MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
표적 설명
19 kDa
물리적 형태
Culture supernatant containing 0.2 M Tris/HCl, pH 7.4 with 5-10% fetal calf serum and 0.1% sodium azide
Unpurified
저장 및 안정성
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
Brain tissue
Brain tissue
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Nucleus-localized 21.5-kDa myelin basic protein promotes oligodendrocyte proliferation and enhances neurite outgrowth in coculture, unlike the plasma membrane-associated 18.5-kDa isoform.
Smith, GS; Samborska, B; Hawley, SP; Klaiman, JM; Gillis, TE; Jones, N; Boggs, JM; Harauz, G
Journal of Neuroscience Research null
Opposing extracellular signal-regulated kinase and Akt pathways control Schwann cell myelination.
Ogata, T; Iijima, S; Hoshikawa, S; Miura, T; Yamamoto, S; Oda, H; Nakamura, K; Tanaka, S
The Journal of Neuroscience null
Induction of oligodendrogenesis in glioblastoma-initiating cells by IFN-mediated activation of STAT3 signaling.
Kanako Yuki, Atsushi Natsume, Hidenori Yokoyama, Yutaka Kondo, Masasuke Ohno et al.
Cancer letters null
Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions.
Fischer, I; Alliod, C; Martinier, N; Newcombe, J; Brana, C; Pouly, S
Testing null
Direct cell-cell interactions control apoptosis and oligodendrocyte marker expression of neuroepithelial cells.
J P Hugnot, K Mellodew, H Pilcher, D Uwanogho, J Price, J D Sinden
Journal of Neuroscience Research null
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.