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Key Documents

MAB367

Sigma-Aldrich

Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3

clone M2-2-B3, Chemicon®, from rat

동의어(들):

Muscarinic Receptor Antibody

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rat

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

M2-2-B3, monoclonal

종 반응성

human, monkey, rat

종 반응성(상동성에 의해 예측)

mammals

제조업체/상표

Chemicon®

기술

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

동형

IgG2a

적합성

not suitable for immunohistochemistry (Paraffin)

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... CHRM2(1129)

특이성

m2 muscarinic acetylcholine receptor. No reactivity with the other subtypes.

SPECIES REACTIVITIES:

Expected to react with most mammalian species.

면역원

i3 loop of m2 receptor fusion protein (225-359), fused to Glutathione S-transferase.

애플리케이션

Anti-Muscarinic Acetylcholine Receptor m2 Antibody, clone M2-2-B3 is an antibody against Muscarinic Acetylcholine Receptor m2 for use in IC, IH & IP.
Immunohistochemistry on 4% paraformaldehyde fixed tissue. Does not work on paraffin embedded tissues. Suggested starting concentration 1-5 μg/mL. It is suggested that you use the PAP system if using this antibody on rat. Immunocytochemistry on transfected cells

Immunoprecipitation Works poorly for immunoblotting Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB367

This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

물리적 형태

Format: Purified
Purified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.

저장 및 안정성

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Distinct balance of excitation and inhibition in an interareal feedforward and feedback circuit of mouse visual cortex.
Yang, W; Carrasquillo, Y; Hooks, BM; Nerbonne, JM; Burkhalter, A
The Journal of Neuroscience null
Heike Schlenz et al.
American journal of physiology. Lung cellular and molecular physiology, 298(5), L626-L636 (2009-12-22)
Cholinergic bronchoconstriction is mediated by M(2) and M(3) muscarinic receptors (MR). In heart and urinary bladder, MR are linked to caveolin-1 or -3, the structural proteins of caveolae. Caveolae are cholesterol-rich, omega-shaped invaginations of the plasma membrane. They provide a
R Nandigama et al.
Neuroscience, 168(3), 842-850 (2010-04-17)
Cell bodies of afferent neurons located in lumbosacral dorsal root ganglia (DRG) provide Adelta- and C-fibres to the urinary bladder, reporting bladder wall tension, volume and noxious stimuli. Recent studies suggested an involvement of muscarinic acetylcholine receptors (mAChRs) not only
Network analysis of corticocortical connections reveals ventral and dorsal processing streams in mouse visual cortex.
Wang, Q; Sporns, O; Burkhalter, A
The Journal of Neuroscience null
W B Carden et al.
The Journal of comparative neurology, 410(3), 431-443 (1999-07-15)
A cholinergic projection from the parabrachial region (PBR) of the brainstem to the visual thalamus has been studied in great detail during the past 20 years. A number of physiological studies have demonstrated that this projection causes a dramatic change

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