추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
55K2, monoclonal
종 반응성
echinoderm, sea urchin, gerbil, human
종 반응성(상동성에 의해 예측)
mammals
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
동형
IgG1
적합성
not suitable for immunoprecipitation
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... FSCN1(6624)
특이성
Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.
면역원
Human fascin
애플리케이션
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.
Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.
Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.
Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.
Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.
Optimal working dilutions must be determined by the end user.
물리적 형태
Format: Purified
Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
저장 및 안정성
Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.
분석 메모
Control
POSITIVE CONTROL:
Hela cells, neuronal tissues, antigen presenting dendritic cells.
POSITIVE CONTROL:
Hela cells, neuronal tissues, antigen presenting dendritic cells.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
S Al-Saad et al.
British journal of cancer, 99(9), 1476-1483 (2008-10-16)
Vimentin, nuclear factor-kappaB (NF-kappaB) p105, fascin, E-cadherin, TGF-beta, Par6 and atypical PKC are molecular markers that play an important role in cell differentiation. Herein, we investigate their prognostic impact in primary non-small-cell carcinoma (NSCLC). Tumour tissue samples from 335 resected
The prognostic impact of TGF-?1, fascin, NF-?B and PKC-? expression in soft tissue sarcomas.
Valkov, A; Sorbye, SW; Kilvaer, TK; Donnem, T; Smeland, E; Bremnes, RM; Busund, LT
Testing null
Protein expression dynamics during postnatal mouse brain development.
Laeremans, A; Van de Plas, B; Clerens, S; Van den Bergh, G; Arckens, L; Hu, TT
Journal of experimental neuroscience null
C S Cohan et al.
Cell motility and the cytoskeleton, 48(2), 109-120 (2001-02-13)
Growth cones at the distal tips of growing nerve axons contain bundles of actin filaments distributed throughout the lamellipodium and that project into filopodia. The regulation of actin bundling by specific actin binding proteins is likely to play an important
S Yamashiro-Matsumura et al.
The Journal of cell biology, 103(2), 631-640 (1986-08-01)
The 55-kD protein is a new actin-bundling protein purified from HeLa cells (Yamashiro-Matsumura, S., and F. Matsumura, 1985, J. Biol. Chem., 260:5087-5097). We have prepared monoclonal antibodies against the 55-kD protein and examined its intracellular localization, as well as its
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