MAB2226

Anti-Piccolo Antibody, clone 4G3.3

clone 4G3.3, from mouse

• 동의어(들): presynaptic cytomatrix protein, Aczonin
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 4G3.3, monoclonal
• 종 반응성: mouse
• 종 반응성(상동성에 의해 예측): human (based on 82% homology), rat (92% homology)
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgMκ
• NCBI 수납 번호: NP_001104266
• UniProt 수납 번호: Q9Y6V0
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PCLO(27445) ; mouse ... Pclo(26875)

설명

일반 설명

Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].

특이성

This antibody recognizes piccolo.

면역원

GST-tagged recombinant protein

애플리케이션

Immunohistochemistry (Paraffin):
Representative images from a previous lot.
Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.

품질

Routinely evaluated by western blot on rat brain lysate.

표적 설명

547 kDa

물리적 형태

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Rat brain lysate, mouse cerebellum

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable