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Merck
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Key Documents

MAB201B

Sigma-Aldrich

Mouse Anti-Rabbit light chain Antibody, biotin conjugate

Chemicon®, from mouse

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.46

생물학적 소스

mouse

Quality Level

결합

biotin conjugate

항체 생산 유형

secondary antibodies

클론

monoclonal

종 반응성

rabbit

제조업체/상표

Chemicon®

기술

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG1κ

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

특이성

Minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep.

애플리케이션

Mouse anti-Rabbit light chain Antibody, biotin conjugate is an antibody against Rabbit light chain for use in ELISA, FC, WB, IH.
Western Blotting: 1:20,000 - 1:400,000
Immunohistochemistry: 1:500 - 1:5,000 on tissue sections
Flow Cytometry: 1:200 - 1:1,000

Optimal working dilutions must be determined by the end user.

표적 설명

25 kDa

물리적 형태

Lyophilized from 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer (IgG-Free, Protease-Free)

저장 및 안정성

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1.1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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