MAB1951Z

Anti-Integrin β1 Antibody, clone P4G11, azide free

clone P4G11, Chemicon^®, from mouse

• 동의어(들): CD29, MAB1951
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: P4G11, monoclonal
• 종 반응성: monkey, human, rabbit
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• 동형: IgG1
• NCBI 수납 번호: NM_002211.2; NM_033666.1; NM_033667.1; NM_033668.1; NM_033669.1; NM_133376.1
• UniProt 수납 번호: P05556
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ITGB1(3688)

설명

특이성

Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.

면역원

Human IL-1beta activated endothelial cells

애플리케이션

Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Traditional formalin fixation is not recommended.

Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)

FACS: 2-5μg per 10e6 cells, in the presence of Ca++.

Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

ELISA

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.

물리적 형태

Format: Purified

Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.

저장 및 안정성

Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable