추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
9D2.2, monoclonal
종 반응성
mouse
제조업체/상표
Chemicon®
기술
ELISA: suitable
western blot: suitable
동형
IgG2b
적합성
not suitable for immunohistochemistry
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ADAM2(2515)
특이성
Recognizes mouse fertilin beta by immunoblot, staining of bands in a 46-48 kDa range (multiple bands are due to variable protein glycosylation).
면역원
Recombinant mouse fertilin produced by baculovirus expressin in SF9 cells.
애플리케이션
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
MMPs & TIMPs
ECM Proteins
MMPs & TIMPs
This Anti-ADAM2 Antibody, clone 9D2.2 is validated for use in ELISA, WB for the detection of ADAM2.
Western blot: 1:500 - 1:1,000 dilution for detected against sperm lysates, using standard alkaline phosphatase with NBT/BCIP detection. Chemiluminescent detection may permit higher dilutions.
Not effective for immunohistochemistry.
Optimal working dilutions must be determined by end user.
Not effective for immunohistochemistry.
Optimal working dilutions must be determined by end user.
물리적 형태
Format: Purified
Liquid at 1 mg/mL in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide
저장 및 안정성
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
H Nishimura et al.
Developmental biology, 233(1), 204-213 (2001-04-26)
We produced mice lacking the sperm surface protein cyritestin (ADAM 3) and found mutant males are infertile. Similar to fertilin beta (ADAM 2) null sperm (C. Cho et al., 1998, Science 281, 1857-1859), cyritestin null sperm are drastically deficient in
Ryo Yamaguchi et al.
Biology of reproduction, 75(5), 760-766 (2006-07-28)
Male mice deficient for the calmegin (Clgn) or the angiotensin-converting enzyme (Ace) gene show impaired sperm migration into the oviduct and loss of sperm-zona pellucida binding ability in vitro. Since CLGN is a molecular chaperone for membrane transport of target
Ryo Yamaguchi et al.
Genes to cells : devoted to molecular & cellular mechanisms, 13(8), 851-861 (2008-09-11)
CD52 is a glycosylphosphatidylinositol (GPI)-anchored antigen expressed on lymphocytes and in epididymal epithelial cells. CD52 is also known as "maturation-associated sperm antigen" but its function is unknown. We therefore generated Cd52 disrupted mice. The resulting Cd52 null mice were healthy
Yasutaka Ueda et al.
The Journal of biological chemistry, 282(42), 30373-30380 (2007-08-23)
A palmitate linked to the inositol in glycosylphosphatidylinositol (GPI) is removed in the endoplasmic reticulum immediately after the conjugation of GPI with proteins in most cells. Previously, we identified PGAP1 (post GPI attachment to proteins 1) as a GPI inositoldeacylase
Kathryn K Stein et al.
Biology of reproduction, 73(5), 1032-1038 (2005-07-15)
Adam2-null and Adam3-null male mice exhibit reduced levels of one or more ADAM proteins on mature sperm, in addition to the loss of the genetically targeted protein. ADAM protein loss was believed to occur posttranslationally, although the timing of loss
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