추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
monoclonal
종 반응성
human, mouse
제조업체/상표
Chemicon®
기술
ELISA: suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... FLT1(2321)
특이성
Recognizes Human Flt-1 (fms-like tyrosine kinase). Flt-1 (human 1338 aa, rat 1336 aa, and mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. Flt-1 has also been described as VEGFR1 and Flk-2. It is type 1 membrane protein and is expressed in normal lung, placenta, liver, kidney, heart, and brain but not in tumor cells (Shibuya et al., 1990; Matsushime et al., 1987; Yamane et al., 1994; Finnerty et al., 1993; Choi et al., 1994; Plouet et al., 1989; Simon et al.,
1998; Sait et al., 1995; deVries et al., 1992).
SPECIES REACTIVITIES:
Antibody crossreacts with mouse Flt-1. No significant sequence homology is seen with human VEGF-R2/KDR or other related kinases. Antibody crossreactivity in other species is not established.
1998; Sait et al., 1995; deVries et al., 1992).
SPECIES REACTIVITIES:
Antibody crossreacts with mouse Flt-1. No significant sequence homology is seen with human VEGF-R2/KDR or other related kinases. Antibody crossreactivity in other species is not established.
면역원
Human recombinant Flt-1 (extracellular domain). Actual epitope recognized by this clone is between 1-250 aa of human Flt-1.
애플리케이션
Anti-VEGF Receptor-1 Antibody is an antibody against VEGF Receptor-1 for use in ELISA & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Western blot: 0.5-2.0 μg/mL. MW = 180 kDa.
Flow cytometry: Dilute the antibody to 25 μg/mL and use 10-20 μL antibody for 1-2 x 10E5 cells in a total volume of 200-250 μL. Visualize with appropriate secondary antibodies.
ELISA: 1:10,000 - 1:100,000, using 50-100ng of control peptide/well.
Optimal working dilutions must be determined by end user.
Flow cytometry: Dilute the antibody to 25 μg/mL and use 10-20 μL antibody for 1-2 x 10E5 cells in a total volume of 200-250 μL. Visualize with appropriate secondary antibodies.
ELISA: 1:10,000 - 1:100,000, using 50-100ng of control peptide/well.
Optimal working dilutions must be determined by end user.
물리적 형태
Format: Purified
Purified from Ascites. Liquid in PBS and 5% Trehalose.
저장 및 안정성
Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze and thaw.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
C de Vries et al.
Science (New York, N.Y.), 255(5047), 989-991 (1992-02-21)
The fms-like tyrosine kinase (Flt) is a transmembrane receptor in the tyrosine kinase family. Expression of flt complementary DNA in COS cells conferred specific, high-affinity binding of vascular endothelial growth factor, also known as vascular permeability factor (VEGF-VPF), a factor
H Matsushime et al.
Japanese journal of cancer research : Gann, 78(7), 655-661 (1987-07-01)
We have isolated a human genomic DNA (designated human frt) cross-hybridizing with the v-ros oncogene of UR2 sarcoma virus. Sequencing analysis of this fragment revealed that this sequence contains a 123-base-pair exon-like structure surrounded by consensus sequences of splice acceptor
Isolation and characterization of a newly identified endothelial cell mitogen produced by AtT-20 cells.
Plouet, J, et al.
The Embo Journal, 8, 3801-3806 (1989)
K Choi et al.
Oncogene, 9(4), 1261-1266 (1994-04-01)
A mouse gene encoding a receptor tyrosine kinase, designated Embryonic receptor kinase (EmRK2), was isolated from embryoid bodies (EBs) generated by differentiating embryonic stem (ES) cells in culture for 6 days. Sequence analysis of EmRK2 cDNA clones predicts a receptor
H Finnerty et al.
Oncogene, 8(8), 2293-2298 (1993-08-01)
A wide range of growth and differentiation processes are regulated by the signalling of receptor tyrosine kinases (RTKs). We have developed a nested polymerase chain reaction (PCR) procedure with degenerate primers, and used it to identify RTKs expressed in murine
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.