추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
BnC6B4, monoclonal
종 반응성
chicken
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ITGA6(3655)
특이성
Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.
면역원
Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6M guanidine isothiocyanate, and depleted of beta1 subunit.
애플리케이션
Anti-Integrin α6 Antibody, clone BnC6B4 is an antibody against Integrin α6 for use in IP, WB, IC, IH.
Western blot
Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor.
Immunohistochemistry: Effective for immunohistochemistry of methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse Ig -FITC secondary antiobody.
Immunocytochemistry: Effective for methanol-fixed cultured cells (15 minute fixation).
Immunoblot: Recognizes a 130 kDa band in chicken brain tissue and a 140 kDa band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).
Optimal working dilutions must be determined by end user.
Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor.
Immunohistochemistry: Effective for immunohistochemistry of methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse Ig -FITC secondary antiobody.
Immunocytochemistry: Effective for methanol-fixed cultured cells (15 minute fixation).
Immunoblot: Recognizes a 130 kDa band in chicken brain tissue and a 140 kDa band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).
Optimal working dilutions must be determined by end user.
물리적 형태
Format: Purified
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Developmentally regulated expression of alpha 6 integrin in avian embryos.
Bronner-Fraser, M, et al.
Development, 115, 197-211 (1992)
Lauren R Strachan et al.
The Journal of cell biology, 167(3), 545-554 (2004-11-03)
Cell migration is essential for proper development of numerous structures derived from embryonic neural crest cells (NCCs). Although the migratory pathways of NCCs have been determined, the molecular mechanisms regulating NCC motility remain unclear. NCC migration is integrin dependent, and
Y Hayashi et al.
The Journal of cell biology, 110(1), 175-184 (1990-01-01)
Chicken integrin beta 1 cDNA and its site-directed mutants were cloned into a mammalian expression vector and introduced into mouse NIH 3T3 cells. Stable transfectants expressing the chicken beta 1 subunit or its site-directed mutants were identified by immunostaining with
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