추천 제품
생물학적 소스
mouse
Quality Level
결합
FITC conjugate
항체 형태
purified immunoglobulin
클론
FMC-7, monoclonal
종 반응성
human
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
동형
IgM
배송 상태
wet ice
타겟 번역 후 변형
unmodified
일반 설명
B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system. FMC-7 B-Cell Lymphocyte Marker is a 105 kDa B cell restricted antigen which is expressed on about 50% of adult human peripheral blood B cells. Upon in vivo B cell activation FMC7 expression initially increases. B cells involved in antibody secretion have lost the FMC7 determinant. The monoclonal antibody FMC7 delineates a subpopulation of B lymphocytes in normal blood. Expression of the antigen recognized by FMC7 appears to be maturation-linked, and it serves to distinguish different types of B cell leukemia.
BIOCHEMISTRY: p I = 7.4
특이성
The FMC-7 antibody was not clustered at the 5th Leucocyte Typing Workshop held in Boston USA 1993. This antibody detects a glycoprotein of 105kD found on circulating B-lymphocytes (Brooks et al., 1981). Studies on normal lymphocytes, leukaemic cells and cell lines indicate that FMC-7 is a marker for a limited segment of the B cell maturation pathway (Catovsky et al., 1981; Zola et al., 1984). This glycoprotein is expressed in varying degrees on normal circulating B-cells, chronic B-cell leukaemia (B-CLL), prolymphocytic leukaemia (PLL) and other B cell neoplasias (Zola et al., 1984, 1987; Bloem et al., 1988; Melo et al., 1988; Ferro & Zola, 1990; Mulligan, 1990; Zola et al., 1991; Burthem & Cawley, 1994; Huh et al., 1994). It labels the same population as CD22 antibodies but reacts with a different antigen (Zola et al. 1987).
Cell reactivity:1
Stains peripheral blood B lymphocytes and tonsil B lymphocytes. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells. Reacts with HRIK and Raji cell lines.
Clinical9,12,13 Expression
B cell prolymphocytic leukemia (B-PLL) Strongly positive
Hairy cell leukemia (HCL) Strongly positive
Hairy cell leukaemia variant (HCL-V) Strongly positive
Splenic lymphoma with villous lymphocytes (SLVL) Positive
B cell chronic lymphocytic leukaemia (B-CLL) Negative to weakly positive
Cell reactivity:1
Stains peripheral blood B lymphocytes and tonsil B lymphocytes. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells. Reacts with HRIK and Raji cell lines.
Clinical9,12,13 Expression
B cell prolymphocytic leukemia (B-PLL) Strongly positive
Hairy cell leukemia (HCL) Strongly positive
Hairy cell leukaemia variant (HCL-V) Strongly positive
Splenic lymphoma with villous lymphocytes (SLVL) Positive
B cell chronic lymphocytic leukaemia (B-CLL) Negative to weakly positive
면역원
The human B-lymphoblastoid cell line, HRIK.
애플리케이션
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This Anti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated is validated for use in Flow Cytometry (FC) for the detection of FMC-7 B-Cell Lymphocyte Marker.
This antibody can be used for the differentiation of PLL from B-CLL. It is also useful in confirmation of the diagnosis of other disorders such as HCL, HCL-V and SLVL. Useful for the detection of residual disease in patients undergoing treatment for these disorders.
SUGGESTED USAGE:
Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
SUGGESTED USAGE:
Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
표적 설명
105 kDa
결합
Replaces: CBL1416F
물리적 형태
The antibody is supplied in 1.0 ml 0.1M Tris-HCl, 0.5M NaCl, 10mM glycine, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
저장 및 안정성
Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
분석 메모
Control
Human peripheral blood lymphocytes, HRIK and Raji cell lines
Human peripheral blood lymphocytes, HRIK and Raji cell lines
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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