콘텐츠로 건너뛰기
Merck
모든 사진(1)

Key Documents

MAB1087-I

Sigma-Aldrich

Anti-Eosinophil Peroxidase Antibody, clone AHE-1

동의어(들):

EPO, EPX

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

AHE-1, monoclonal

분자량

calculated mol wt 81.04 kDa

정제법

using protein G

종 반응성

human

포장

antibody small pack of 100 μg

기술

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

동형

IgG1κ

에피토프 서열

Unknown

단백질 ID 수납 번호

UniProt 수납 번호

저장 온도

2-8°C

유전자 정보

human ... EPX(8288)

일반 설명

Eosinophil peroxidase (UniProt: P11678; also known as EC:1.11.1.7, EPO, EPX) is encoded by the EPX (also known as EPER, EPO, EPP) gene (Gene ID: 8288) in human. Eosinophil peroxidase is an enzyme found in cytoplasmic granules of eosinophils. It mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. It is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 18-139), which are subsequently cleaved off to produce the mature form that can be cleaved into a light chain (aa 140-250) and a heavy chain (aa 251-715). These chains can assemble to for a tetrameric structure. It is a heme-containing enzyme that physically associates with fibrous tissue and cancer tissue in various organs. It contains well-conserved profibrogenic capacity to stimulate the migration of fibroblastic cells and promote their ability to secrete collagenous proteins to generate a functional extracellular matrix (ECM) both in vitro and in vivo. It plays a role in regulating the recruitment of fibroblast and the biosynthesis of collagen ECM at sites of normal tissue repair and fibrosis. It is a key participant in generation of reactive oxidants and diffusible radical species by the phagocytes. It serves as a potent toxin towards invading parasites and the surrounding tissues. It also displays significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. (Ref.: DeNichilo, MO., et al. (2015). Am. J. Pathol. 185(5); 1372-1384; Mitra, SN., et al. (2000). Redox Rep. 5(4); 215-224).

특이성

Clone AHE-1 is a mouse monoclonal antibody that detects Eosinophil Peroxidase.

면역원

Human Eosinophils from a patient with hypereosinophilic syndrome.

애플리케이션

Quality Control Applications

Evaluated by Immunofluorescence in frozen Human colon tissue sections.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected Eosinophil Peroxidase in frozen Human colon tissue sections.

Tested applications

Immunofluorescence Analysis: A representative lot detected Eosinophil Peroxidase in Immunofluorescence applications (DeNichilo, M.O., et al. (2015). Am J Pathol. 185(5); 1372-84; Song, Y., et al. (2013). ISRN Inflamm. 2013; 907821).

Immunohistochemistry Applications: A representative lot detected Eosinophil Peroxidase in Immunohistochemistry applications (Spang, C., et al. (2017). J Musculoskelet Neuronal Interact. 17(3); 226-236; Song, Y., et al. (2013). ISRN Inflamm. 2013; 907821; Song, Y., et al. (2013). BMC Musculoskelet Disord. 14:; 34; Song, Y., et al. (2012). PLoS One ;7(12); e52230).

Immunocytochemistry Analysis: A representative lot detected Eosinophil Peroxidase in Immunocytochemistry applications (DeNichilo, M.O., et al. (2015). Am J Pathol. 185(5); 1372-84).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.