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Merck
모든 사진(1)

주요 문서

MAB10764

Sigma-Aldrich

Anti-SBP-tag Antibody, clone 20 | MAB10764

clone 20, 1.0 mg/mL, from mouse

동의어(들):

streptavidin binding peptide

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

20, monoclonal

종 반응성

human

농도

1.0 mg/mL

기술

immunocytochemistry: suitable
western blot: suitable

동형

IgG1κ

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

Streptavidin Binding Peptide tag is a useful and versatile peptide that can be inserted into expression vectors for easy detection of recombinant proteins and purification. The sequence for SBP-tag is 38 amino acids long having a nanomolar scale binding affinity for streptavidin with an equilibrium constant of 2.5 nM.

특이성

Demonstrated to react with cells expressing the SBP tag.
This antibody recognizes SBP tag.

면역원

Epitope: Unknown
Linear peptide corresponding to SBP tag.

애플리케이션

Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hudson, D., et al. (2008). MBC. 19(7):3070-3079.)
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

품질

Evaluated by Western Blot in pTrAP-GFP transfected Hek293 lysate.

Western Blot Analysis: 0.1 ng/mL of this antibody detected SBP-tag on 10 µg of pTrAP-GFP transfected Hek293 lysate.

표적 설명

The protein expresss is pTrAP-GFP plasmids containing a Histidine tag, S-tag, streptavidin-binding pepide (SBP) tag, and GFP. The protein expressing is ~37 kDa

물리적 형태

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
pTrAP-GFP transfected Hek293 lysate

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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Molecular biology of the cell, 19(7), 3070-3079 (2008-05-16)
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