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Merck
모든 사진(4)

Key Documents

MAB1072

Sigma-Aldrich

Anti-Erythropoietin Antibody, clone 1C8

clone 1C8, Chemicon®, from mouse

동의어(들):

erythropoietin, Epoetin, epoetin

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

클론

1C8, monoclonal

종 반응성

human

제조업체/상표

Chemicon®

기술

ELISA: suitable
immunofluorescence: suitable
western blot: suitable

동형

IgG2b

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.

특이성

Reacts with human Erythropoietin (Epo).

면역원

Recombinant human Epo.

애플리케이션

Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.
ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.

Immunosorbent preparation

Optimal working dilutions must be determined by the end user.
Research Category
Metabolism
Research Sub Category
Metabolic Hormones & Receptors

품질

Evaluated by Western Blot on Mouse kidney lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.

표적 설명

34 kDa (glycosylated)

물리적 형태

Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

저장 및 안정성

Stable for 1 year at 2-8ºC from date of receipt.

분석 메모

Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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