MAB10207-I

Anti-Fusion Glycoprotein of HPIV 3 Antibody

• UNSPSC 코드: 12352203

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 9-4-3, monoclonal
• 분자량: calculated mol wt 60.06 kDa
• 종 반응성: virus
• 포장: antibody small pack of 100 μL
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1
• UniProt 수납 번호: P06828
• 배송 상태: dry ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NEWENTRY(2828653)

설명

일반 설명

Fusion glycoprotein F0 (UniProt: P06828) is encoded by the F gene (Gene ID: 2828653) by the Human parainfluenza 3 virus (HPIV-3). HPIVs are antigenically divided into types 1-4 and type 1 and 3 are reported to cause most lower respiratory infections. HPIVs are enveloped and of medium size (150 to 250 nm), and their RNA genome is in the negative sense. HPIV3 displays three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide near the C-terminal region of the ectodomain. The formation of this structure drives apposition and subsequent fusion of viral and plasma cell membranes. This fusion is reported to be pH independent and occurs directly at the outer cell membrane. Fusion glycoprotein F0 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form. This inactive precursor F0 is glycosylated and proteolytically cleaved into F1 (aa 110-539) and F2 (19-109) to be functionally active. The cleavage is mediated by cellular proteases during the transport and maturation of the polypeptide. (Ref.: Hendrickson, KJ. (2003). Clin. Microbiol. Rev. 16(2); 242-264).

특이성

Clone 9-4-3 is mouse monoclonal antibody that detects Fusion glycoprotein of Parainfluenza 3 virus.

면역원

Fusion (F) glycoprotein of human parainfluenza type 3 virus.

애플리케이션

Quality Control Testing

Evaluated by Immunocytochemistry in LLC-ML2 cells infected with HPIV-3.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Fusion Glycoprotein of HPIV 3 in LLC-ML2 cells infected with HPIV-3.

Tested Applications

Western Blotting Analysis: A representative lot detected Fusion Glycoprotein of HPIV-3 in Western Blotting applications (Xie, W., et. al. (2015). PLoS One. 10(8):e0136474).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Fusion Glycoprotein of HPIV 3, clone 9-4-3, Cat. No. MAB10207-I, is a mouse monoclonal antibody that detects human parainfluenza type 3 virus and is tested for use in Immunocytochemistry and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at +2°C to +8°C from date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1