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Merck
모든 사진(1)

주요 문서

IM16

Sigma-Aldrich

Anti-Cathepsin D (Ab-2) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC 코드:
12352203

생물학적 소스

rabbit

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

liquid

포함

≤0.1% sodium azide as preservative

종 반응성

human

제조업체/상표

Calbiochem®

저장 조건

do not freeze

동형

IgG

배송 상태

wet ice

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... CTSD(1509)

일반 설명

Anti-Cathepsin D (Ab-2), rabbit polyclonal, recognizes the ~52 kDa latent form and ~34 kDa subunit in breast carcinoma tissue. It is validated for WB and IHC with frozen and paraffin sections.
Purified rabbit polyclonal antibody. Recognizes the ~52 kDa latent form and the ~34 kDa (but not the ~14 kDa) active subunit of cathepsin D.
Recognizes the ~52 kDa latent form D and the ~34 kDa subunit, but not the ~14 kDa subunit of cathepsin D in breast carcinoma tissue extracts.

면역원

Human
purified human liver cathepsin D

애플리케이션

Frozen Sections (5 µg/ml)

Immunoblotting (2.5 µg/ml)

Paraffin Sections (5 µg/ml, trypsin pre-treatment required)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

물리적 형태

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

분석 메모

Positive Control
HT1080 concentrated conditioned medium or breast carcinoma tissue

기타 정보

For optimal results in immunoblotting, conditioned media from HT1080 cells should be concentrated 10-20X prior to loading. Antibody should be titrated for optimal results in individual systems.
Rochefort, H. 1992. Eur. J. Cancer28A, 1780.
Rochefort, H. 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell20, 352.
Barret, A. J. 1977. In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10-13 - German Storage Class 10 to 13


시험 성적서(COA)

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문서 라이브러리 방문

S N Wickramasinghe et al.
British journal of haematology, 101(2), 245-250 (1998-06-03)
Ultrastructural immunocytochemical studies were performed on sections of bone marrow from three patients with beta-thalassaemia major and two patients with haemoglobin H (HbH) disease. Some sections were reacted with either a polyclonal or a monoclonal anti-human-ubiquitin antibody and the reaction
Vaishnavi Venugopalan et al.
International journal of molecular sciences, 22(11) (2021-06-03)
Cathepsin K-mediated thyroglobulin proteolysis contributes to thyroid hormone (TH) liberation, while TH transporters like Mct8 and Mct10 ensure TH release from thyroid follicles into the blood circulation. Thus, thyroid stimulating hormone (TSH) released upon TH demand binds to TSH receptors
Espen Melum et al.
Nature immunology, 20(12), 1644-1655 (2019-10-23)
Invariant natural killer T (iNKT) cells recognize activating self and microbial lipids presented by CD1d. CD1d can also bind non-activating lipids, such as sphingomyelin. We hypothesized that these serve as endogenous regulators and investigated humans and mice deficient in acid
Seung-Il Choi et al.
Autophagy, 8(12), 1782-1797 (2012-09-22)
Granular corneal dystrophy type 2 (GCD2) is an autosomal dominant disease characterized by a progressive age-dependent extracellular accumulation of transforming growth factor β-induced protein (TGFBI). Corneal fibroblasts from GCD2 patients also have progressive degenerative features, but the mechanism underlying this
Petr Vodicka et al.
Journal of Huntington's disease, 5(3), 249-260 (2016-10-01)
Mutant huntingtin (mHTT) is encoded by the Huntington's disease (HD) gene and its accumulation in the brain contributes to HD pathogenesis. Reducing mHTT levels through activation of the autophagosome-lysosomal pathway may have therapeutic benefit. Transcription factor EB (TFEB) regulates lysosome

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