GR13L

Anti-EGFR (Ab-3) Mouse mAb (225)

lyophilized, clone 225, Calbiochem^®

• 동의어(들): Anti-EGF-R
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 225, monoclonal
• 형태: lyophilized
• 미포함: preservative
• 종 반응성: human
• 반응하면 안 됨: rat, mouse
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 동형: IgG1
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

설명

일반 설명

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.

Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.

면역원

Human

partially purified, human EGFR from A431 cells

애플리케이션

Immunoprecipitation (1-2 µg per sample)

Neutralization Studies (2 µg/ml, see comments and application references)

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.

재구성

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

분석 메모

Negative Control
HL-60 cells

Positive Control
A431 cells

기타 정보

Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable