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Merck
모든 사진(1)

주요 문서

GF35L

Sigma-Aldrich

Anti-BDNF Mouse mAb (35928.11)

lyophilized, clone 35928.11, Calbiochem®

동의어(들):

Anti-Brain Derived Neurotrophic Factor

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

35928.11, monoclonal

양식

lyophilized

미포함

preservative

종 반응성

human

제조업체/상표

Calbiochem®

저장 조건

OK to freeze
avoid repeated freeze/thaw cycles

동형

IgG1

배송 상태

ambient

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... BDNF(627)

일반 설명

Anti-BDNF, mouse monoclonal, clone 35928.11, recognizes the ~20 kDa BDNF protein in brain extracts. It is validated for ELISA, Western blotting, immunohistochemistry, and neutralization studies.
Immunoaffinty purified mouse monoclonal antibody. Recognizes the ~20 kDa BDNF protein.
Recognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.

면역원

recombinant protein consisting of amino acids 129-247 of human BDNF

애플리케이션

ELISA (0.5-1 µg/ml)

Immunoblotting (1-2 µg/ml)

Immunohistochemistry (5-15 µg/ml)

Neutralization Studies (see comments)

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from PBS, 5% trehalose.

재구성

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).

기타 정보

Riccio, A., et al. 1997. Science277 1097.
Sendtner, M., et al. 1996. Neurochem. Res.21, 831.
Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442.
Gotz, R., et al. 1994. Nature372, 266.
Snider, W.D. 1994. Cell77, 627.
Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.
The full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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