GF156

B18R, Human Recombinant Carrier-Free

liquid, >98% (SDS-PAGE), <0.05 ng/μg cytokine (LAL test)

• 동의어(들): B18R protein, Recombinant B18R
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

속성

• 생물학적 소스: human
• Quality Level: 100
• 분석: >98% (SDS-PAGE)
• 양식: liquid
• 특이 활성도: ≥1.3 x 10e6 units/mg protein
• 분자량: M_w 38,389 Da
• 농도: 0.5 mg/mL
• 기술: cell culture | stem cell: suitable
• 불순물: <0.05 ng/μg cytokine (LAL test)
• 입력: sample type induced pluripotent stem cell(s)
• solubility: water: soluble
• 적합성: suitable for molecular biology
• 배송 상태: dry ice

설명

일반 설명

Product Source: Insect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.

Research area: Immuno and CKS B18R is a virally-encoded protein that acts as a decoy receptor for Type 1 Interferons. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta.

애플리케이션

B18R, Human RecombinantCarrier-Free has been used as a decoy receptor to study the intrinsic antiviralactivity of recombinant soluble IFNα/β cell surface receptor 2 (sIFNAR2). Ithas also been used in the generation and maintenance of induced pluripotentstem cells (iPSCs) and human IPSCs.

물리적 형태

Sterile liquid;10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer and is filtered with a 0.2µm filter before vialing.

저장 및 안정성

For best recovery, quick-spin vial prior to opening. Thaw on ice. While on ice, aliquot B18R protein into sterile, nuclease-free, low protein-binding eppendorf tubes and store at -80°C. Limit repeated freeze and thaw cycle. Use in a sterile environment.
Further dilutions should be made into appropriate buffer or medium.

분석 메모

The DTT-reduced protein migrates as a 48 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a 46 kDa polypeptide on SDS-PAGE due to internal cystines.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable