FCMAB113A4

Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor^™ 488 conjugate

clone 10H11.2, from mouse, ALEXA FLUOR^™ 488

• 동의어(들): Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: ALEXA FLUOR^™ 488
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 10H11.2, monoclonal
• 종 반응성: human
• 기술: flow cytometry: suitable; immunocytochemistry: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_002701.4
• UniProt 수납 번호: Q01860
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... POU5F1(5460)

설명

일반 설명

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

특이성

Reacts with human Oct-4.

면역원

Recombinant GST fusion protein, human Oct-4 (aa 1-134).

애플리케이션

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.

Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments

Working Solution	Cells / test	Total Reaction Volume
5 μl	5 x 105 Cells / 95 μl PBS	100 μl
10 μl	1 x 106 Cells / 90 μl PBS	100 μl

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

품질

Evaluated by Flow Cytometry in 2102Ep Cells.

표적 설명

~44 kDa

물리적 형태

Purified mouse monoclonal IgG1 conjugated to Alexa Flour 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.

저장 및 안정성

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt

분석 메모

Control
Human embryonic stem cells

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable