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Merck
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Key Documents

ELI-008-M

Sigma-Aldrich

IL-6 ELISPOT Antibody Pair, Mouse

from rat, clone MP5-20F3, clone MP5-32C11

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rat

Quality Level

클론

MP5-20F3, monoclonal
MP5-32C11, monoclonal

종 반응성

mouse

기술

ELISA: suitable

배송 상태

wet ice

일반 설명

Interleukin 6 (IL-6) is a multifunctional cytokine that plays an important role in regulating immune and inflammatory responses. Produced by a wide variety of cells, including T cells, monocytes, fibroblasts, endothelial cells, and keratinocytes, IL-6 works by stimulating B-cell differentiation and antibody production, as well inducing expression of hepatic acute-phase proteins, regulating bone metabolism, and promoting neuronal differentiation. In addition, IL-6 appears to interact with IL-2 in the proliferation of T-lymphocytes and with IL-3 to promote the proliferation of multipotent hematopoietic progenitor cells. The Millipore mouse IL-6 ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of IL-6 secreting cells at the single cell level.

특이성

Both the capture and the detection antibodies included in this kit react with mouse interleukin -6 (IL-6).

애플리케이션

IL-6 ELISPOT Antibody Pair, Mouse is a highly specific rat monoclonal antibody & that targets IL-6 & has been tested in ELISA.
Research Category
Inflammation & Immunology

포장

Sufficient reagents for five, 96-well plates.

성분

• Mouse IL-6 Capture Antibody, clone MP5-20F3. Quantity: 500 µg.

• Mouse IL-6 Detection Antibody, clone MP5-32C11. Quantity: 100 µg.

• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.

표적 설명

~25 kDa

물리적 형태

Format: Purified
The 500 µg purified IL-6 capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and the 100 µg purified IL-6 biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.

저장 및 안정성

Maintain refrigerated at 2-8°C for up to six months from the date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

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문서 라이브러리 방문

문서

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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