추천 제품
종 반응성
vertebrates
Quality Level
포장
pkg of 1 kit
제조업체/상표
Chemicon®
기술
ELISA: suitable
배송 상태
dry ice
저장 온도
2-8°C
일반 설명
The CHEMICON in vitro angiogenesis assay kit represents a simple model of angiogenesis in which the induction or inhibition of tube formation by exogenous signals can be easily monitored. Angiogenesis is the process of generating new capillary blood vessels. It is a fundamental component of several normal (reproduction and wound healing) and pathological processes (diabetic retinopathy, rheumatoid arthritis, tumor growth, and metastasis).
The CHEMICON in vitro angiogenesis assay kit provides a convenient system for evaluation of tube formation by endothelial cells in a convenient 96-well format. When cultured on ECMatrix, a solid gel of basement proteins prepared from the Engelbreth Holm-Swarm (EHS) mouse tumor, these endothelial cells rapidly align and form hollow tube-like structures. Tube formation is a multi-step process involving cell adhesion, migration, differentiation, and growth.
ECMatrix is optimized for maximal tube-formation. For assaying inhibitors or stimulators of tube formation, simply premix the endothelial cell suspension with different concentrations of the inhibitor or stimulator to be tested, before adding the cells to the top of the ECMatrix.
The CHEMICON in vitro angiogenesis assay kit provides a convenient system for evaluation of tube formation by endothelial cells in a convenient 96-well format. When cultured on ECMatrix, a solid gel of basement proteins prepared from the Engelbreth Holm-Swarm (EHS) mouse tumor, these endothelial cells rapidly align and form hollow tube-like structures. Tube formation is a multi-step process involving cell adhesion, migration, differentiation, and growth.
ECMatrix is optimized for maximal tube-formation. For assaying inhibitors or stimulators of tube formation, simply premix the endothelial cell suspension with different concentrations of the inhibitor or stimulator to be tested, before adding the cells to the top of the ECMatrix.
애플리케이션
In vitro angiogenesis assay kit has been used to monitor the extent of tube assembly/angiogenic potential in various endothelial cells such as:
- human umbilical vein cells (HUVEC)
- primary human retinal microvascular cells
- platelet derived growth factor receptor-β (PDGFRβ)/PAE cells
- bovine capillary endothelial (BCE) cells
성분
- ECMatrixTM Gel Solution: (Part No. 90060) Five 1 mL bottles.
- ECMatrixTM Diluent Buffer, 10X: (Part No. 90061) One 1 mL bottle.
저장 및 안정성
Store kit materials at -20 ºC for up to 6 months. Once ECMatrix has been thawed, store at 2-8 ºC for one week. Do not refreeze.
기타 정보
The CHEMICON in vitro angiogenesis assay kit is intended for research use only; not for diagnostic or therapeutic applications.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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