콘텐츠로 건너뛰기
Merck
모든 사진(1)

Key Documents

ECM205

Sigma-Aldrich

Millicoat® ECM Screening Kit, 1 ea. ECM101-ECM105

Millicoat®, pkg of  96-well plate(s) (for fibronectin, vitronectin, laminin, collagen I & collagen IV)

동의어(들):

Formerly under the CytoMatrix brand name.

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352202
eCl@ss:
32161000
NACRES:
NA.77

종 반응성

human

Quality Level

제조업체/상표

Chemicon®
Millicoat®

포장

pkg of  96-well plate(s) (for fibronectin, vitronectin, laminin, collagen I & collagen IV)

기술

activity assay: suitable
cell based assay: suitable

입력

sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)

검출 방법

colorimetric

배송 상태

wet ice

일반 설명

Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat® Cell Adhesion Strips are provided as 12 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with a human ECM protein. Row H of each strip is coated with BSA which serves as a negative assay control. The Millicoat screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I and collagen IV. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.

애플리케이션

PROCEDURE:

NOTE: Optimal assay performance is obtained using subconfluent cell cultures. This can be achieved by splitting the cells 1 to 2 days prior to performing the assay.

1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydated strips.

2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.

3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 3 times with PBS containing Ca2+/Mg2+ (200 mL/well).

4. Add 100 mL/well of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).

5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.

6. Determine the absorbances at 540 - 570 nm on a microplate reader.
Research Category
Cell Structure
The Millicoat® screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I & collagen IV.

저장 및 안정성

May be stored at 2-8°C in the foil pouch for at least 3 months. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLICOAT is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Tomonori Kaneda et al.
Cancer letters, 270(2), 354-361 (2008-07-09)
This study focused on the role of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase important for many cellular processes, in the proliferation, adhesion, and invasion of melanoma cells in vitro and in vivo. We found that the Y925F-mutation of
K E Crawford et al.
Placenta, 33(5), 416-423 (2012-03-02)
Calreticulin is a calcium binding, endoplasmic reticulum resident protein best known for its roles in intracellular calcium homeostasis and the quality control processes of the endoplasmic reticulum. There is evidence for a range of activities for calreticulin outside the endoplasmic
Rosalyn D Blumenthal et al.
Cancer research, 65(19), 8809-8817 (2005-10-06)
CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. The effects of three monoclonal antibodies targeting different epitopes on these antigens (NH2-terminal [MN-3] and A1B1 domains [MN-15] shared by CEACAM5 and CEACAM6 and the
Guangchun Sun et al.
Journal of Cancer, 6(10), 996-1004 (2015-09-15)
Hepatocellular carcinoma (HCC) is a type of malignant cancer. Notch signaling is aberrantly expressed in HCC tissues with more evidence showing that this signaling plays a critical role in HCCs. In the present study, we investigate the effects of the
Mary Ann Stepp et al.
Journal of cell science, 120(Pt 16), 2851-2863 (2007-08-02)
We have reported previously that syndecan-1 (Sdc1)-null mice show delayed re-epithelialization after skin and corneal wounding. Here, we show that primary keratinocytes obtained from Sdc1-null mice and grown for 3-5 days in culture are more proliferative, more adherent and migrate

문서

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

관련 콘텐츠

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.