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Merck
모든 사진(1)

Key Documents

CP70

Sigma-Aldrich

Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)

liquid, clone FBN11, Calbiochem®

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.43

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

FBN11, monoclonal

형태

liquid

포함

≤0.1% sodium azide as preservative

종 반응성

rat, human, mouse

제조업체/상표

Calbiochem®

저장 조건

do not freeze

동형

IgG1

배송 상태

wet ice

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... FN1(2335)
mouse ... Fn1(14268)
rat ... Fn1(25661)

일반 설명

Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.
Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.

면역원

recombinant human fibronectin

애플리케이션

Frozen Sections (0.5-1 µg/ml)

Immunoblotting (0.5-1 µg/ml)

Immunofluorescence (see comments)

Immunoprecipitation (2 µg/mg protein lysate)

Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

분석 메모

Positive Control
SW 156 cells or normal kidney tissue

기타 정보

Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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Journal of thrombosis and haemostasis : JTH, 5(10), 2070-2078 (2007-07-26)
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Ismaïl Hendaoui et al.
Matrix biology : journal of the International Society for Matrix Biology, 40, 46-53 (2014-09-10)
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Osteosarcoma is the most common primary malignancy of the bone and is characterized by local invasion and distant metastasis. Over the past 20 years, long-term outcomes have reached a plateau even with aggressive therapy. Overexpression of insulin-like growth factor 1 receptor
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