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Merck
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Key Documents

CBL440

Sigma-Aldrich

Anti-c-Fos Antibody

CHEMICON®, sheep polyclonal

동의어(들):

G0/G1 Switch Regulatory Protein 7

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-c-Fos Antibody, Chemicon®, from sheep

생물학적 소스

sheep

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

human, rat

제조업체/상표

Chemicon®

기술

immunocytochemistry: suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... FOS(2353)

특이성

c-fos is a 55 kDa nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents. c-fos is also induced in neuronal tissue following drug induced seizures.

KNOWN SPECIES CROSS REACTIVITY: Known to cross-react with rat

면역원

Synthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala-Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos.

애플리케이션

Anti-c-Fos Antibody is a Sheep Polyclonal Antibody for detection of c-Fos also known as G0/G1 Switch Regulatory Protein 7 & has been validated in WB, ICC.
Immunoblotting: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein. CBL 440 detects a band at 55-60 kDa on immunoblots of stimulated HeLa and HL-60 lysates.

Immunocytochemistry: CBL 440 has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (where CBL 440 detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment.

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

물리적 형태

Format: Purified
The purified immunoglobulin is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

저장 및 안정성

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

S P Hunt et al.
Nature, 328(6131), 632-634 (1987-08-13)
It has been suggested that the proto-oncogenes c-fos and c-myc participate in the control of genetic events which lead to the establishment of prolonged functional changes in neurons. Expression of c-fos and c-myc are among the earliest genetic events induced
Transcription activation by serum, PDGF, and TPA through the c-fos DSE: cell type specific requirements for induction.
Siegfried, Z and Ziff, E B
Oncogene, 4, 3-11 (1989)
Effects of epidermal growth factor and platelet-derived growth factor on c-fos and c-myc mRNA levels in normal human fibroblasts.
Paulsson, Y, et al.
Experimental Cell Research, 171, 186-194 (1987)
Dissociation of c-fos induction from macrophage differentiation in human myeloid leukemic cell lines.
Calabretta, B
Molecular and cellular biology, 7, 769-774 (1987)
Long-lasting and sequential increase of c-fos oncoprotein expression in kainic acid-induced status epilepticus.
Le Gal La Salle, G
Neuroscience Letters, 88, 127-130 (1988)

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