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Merck
모든 사진(1)

주요 문서

CBL419

Sigma-Aldrich

Anti-Eosinophil Major Basic Protein Antibody, clone BMK13

clone BMK13, Chemicon®, from mouse

동의어(들):

EMBP

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

BMK13, monoclonal

종 반응성

human

반응하면 안 됨

horse, mouse, rat

제조업체/상표

Chemicon®

기술

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

동형

IgG1

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... PRG2(5553)

특이성

CBL419 is specific for eosinophil major basic protein irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreated pro-form of MBP (Pregnancy Associated MBP) found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granual. It does not cross-react with horse, mouse or rat MBP.

애플리케이션

Anti-Eosinophil Major Basic Protein Antibody, clone BMK13 is an antibody against Eosinophil Major Basic Protein for use in ELISA, WB, IH(P).
Immunohistochemistry: Clone BMK-13 reacts with fresh frozen tissues that were sectioned (6μm), dried for 1 hour at room temperature and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium foil at -70°C until used. For paraffin embedded sections, 4% PFA (3hr) should be used for skin biopsies. 5 μM sections are dewaxed, and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues.
Western blotting: purified normal human eosinophils were treated with digitonin (5mg/mL) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~ 14kDa, and typical human samples range from 10-16kDa.
ELISA development for MBP estimation.

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

물리적 형태

Format: Purified
The monoclonal is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

저장 및 안정성

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Age-related changes in proliferation, the numbers of mast cells, eosinophils, and cd45-positive cells in human dermis.
Gunin AG, Kornilova NK, Vasilieva OV, Petrov VV
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences null
Takahiro Kawakami et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 34(6), 960-969 (2018-10-17)
This study aimed to investigate the contribution of a proliferation-inducing ligand (APRIL), a member of the tumor necrosis factor (TNF) superfamily implicated in plasma cell survival, to the development of plasma cell-rich lesions in immunoglobulin G4-related disease (IgG4-RD). We performed
Toru Sonoyama et al.
International journal of molecular sciences, 23(21) (2022-11-12)
Transglutaminase (TGM) isoform catalyze the cross-linking reaction of identical or different substrate proteins. Eosinophil has been recognized in chronic rhinosinusitis with nasal polyps (CRSwNP) forming tissue eosinophil in nasal polyp (NP), and TGM isoforms are suggested to be associated with
Hironobu Nishijima et al.
Laboratory investigation; a journal of technical methods and pathology, 96(9), 981-993 (2016-06-21)
The posterior nasal nerve is the dominant source of the parasympathetic, sympathetic, and sensory fibers that innervate the nasal respiratory mucosa. Therefore, a posterior nasal neurectomy (PNN) is thought to induce denervation of the nasal mucosa and relieve the nasal

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