CBL150-I

Anti-CD3e Antibody, clone UCHT1

clone UCHT1, from mouse

• 동의어(들): T-cell surface glycoprotein CD3 epsilon chain, CD3-epsilon, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: UCHT1, monoclonal
• 종 반응성: human
• 기술: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_000724
• UniProt 수납 번호: P07766
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CD3E(916)

설명

일반 설명

T-cell surface glycoprotein CD3 epsilon chain (UniProt P07766; also known as CD3-epsilon, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain) is encoded by the CD3E (also known as T3E) gene (Gene ID 916) in human. The T cell receptor or TCR is a plasma membrane protein complex responsible for recognizing antigens bound to major histocompatibility complex (MHC) on antigen presenting cells. In 95% of T cells, the TCR is composed of a disulfide-linked alpha (α) and beta (β) dimer, while among 5% of T cells the TCR is composed of a gamma (γ) and a delta (δ) dimer. T cells are activated when their surface TCRs engage with antigen/MHC complex on antigen presenting cells. Each TCR alpha and beta chain contains a variable (V) and a constant (C) extracellular immunoglobulin-like domain, followed by a transmembrane region and a short cytoplasmic tail. The alpha and beta chains have very short cytoplasmic tail and rely on the CD3 (cluster of differentiation 3) co-receptor complex for transducing signaling further intracellularly. The CD3 co-receptor complex is composed of a CD3γ chain, a CD3δ chain, and two CD3ε chains. The CD3 complex, TCR dimer, and a ζ-chain together comprise the functional TCR complex. The CD3 epsilon chain is initially produced with a signal peptide (a.a. 1-22), the removal of which yields the mature chain (a.a. 23-207) with an Ig-like (a.a. 32-112) domain-containing extracellular region (a.a. 23-126), a transmembrane segment (a.a. 127-152), and a cytoplasmic tail (a.a. 153-207), which contains the immunoreceptor tyrosine-based activation motif or ITAM (a.a. 178-205) that is essential for mediating ZAP-70 interaction and transducing TCR signaling.

특이성

Clone UCHT1 reacts with the extracellular domain of human CD3 epsilon chain (Clevers, H., et al. (1988). Eur. J. Immunol. 18(5):705-710).

면역원

Epitope: Extracellular domain.

Human infant thymocytes and lymphocytes from a patient with Sézary disease.

애플리케이션

Anti-CD3e Antibody, clone UCHT1 is an antibody against CD3e for use in Flow Cytometry, Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry, Affects Function.

Immunoprecipitation Analysis: Representative lots immunoprecipitated CD3 epsilon-associated TCR complex (Schamel, W.W., et al. (2005). J. Exp. Med. 202(4):493-503; Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
ELISA Analysis: A representative lot was used as the capture antibody for the detection of epsilon-associated TCR complex proteins by sandwich ELISA (Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
Immunocytochemistry Analysis: A representative lot detected the exogensously expressed human CD3 epsilon chain on the surface of murine T cells by flourescent immunocytochemistry (Clevers, H., et al. (1988). Eur. J. Immunol. 18(5):705-710).
Immunocytochemistry Analysis: A representative lot was employed to determined the plasma membrane and cytoplasmic CD3 immunoreactivity in T cell populations and T-ALL blast cells by flourescent immunocytochemistry (Campana, D., et al. (1987). J. Immunol. 138(2):648-655).
Immunohistochemistry Analysis: A representative lot detected CD3+ T cells (T3+) in the periglandular stromal aggregates in frozen dometrium sections from female donors undergone routine dilation and curettage procedures (Kamat, B.R., and Isaacson, P.G. (1987). Am. J. Pathol. 127(1):66-73).
Affects Function: A representative lot induced surface IL-2R expression and proliferation of human peripheral blood T lymphocytes (Van Wauwe, J.P., et al. (1984). J. Immunol. 133(1):129-132).
Flow Cytometry Analysis: A representative lot was employed for fluorescence activated cell sorting (FACS) of CD3+ human lymphocytes (Beverley, P.C., and Callard, R.E. (1981). Science. 11(4):329-334).

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

품질

Evaluated by Flow Cytometry in human PBMC.

Flow Cytometry Analysis: 0.1 µg of this antibody detected CD3e in 1X10E6 human PBMC.

표적 설명

23.15 kDa (Proform) and 20.74 (mature form) calculated. ~27 kDa reported in UCHT1 immunoprecipitate (Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in PBS without preservatives.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable