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Merck
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Key Documents

AP501B

Sigma-Aldrich

Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed

Chemicon®, from goat

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.46

생물학적 소스

goat

Quality Level

결합

biotin conjugate

항체 형태

affinity purified immunoglobulin

항체 생산 유형

secondary antibodies

클론

polyclonal

종 반응성

mouse

제조업체/상표

Chemicon®

기술

ELISA: suitable
immunofluorescence: suitable

배송 상태

wet ice

타겟 번역 후 변형

unmodified

일반 설명

IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

특이성

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

면역원

Epitope: Mouse IgM, IgG, IgA
Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.

애플리케이션

Detect Mouse IgM+IgG+IgA (H+L) using this Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed validated for use in ELISA & IF.
Immunofluorescence: ≤1 μg/10E6 cells
ELISA: 1:5,000-1:20,000

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

물리적 형태

Affinity Chromatography
Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.

저장 및 안정성

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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