AP192B

Donkey Anti-Mouse IgG Antibody, biotin conjugate, Species Adsorbed

0.8 mg/mL (after reconstitution), Chemicon^®

• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

속성

• 생물학적 소스: donkey
• Quality Level: 100
• 결합: biotin conjugate
• 항체 형태: F(ab′)₂ fragment of affinity isolated antibody
• 항체 생산 유형: secondary antibodies
• 클론: polyclonal
• 종 반응성: mouse
• 제조업체/상표: Chemicon^®
• 농도: 0.8 mg/mL (after reconstitution)
• 기술: ELISA: suitable; western blot: suitable
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

특이성

Specific for mouse IgG, heavy and light chain.

애플리케이션

Donkey anti-Mouse IgG Antibody, biotin conjugate, Species Adsorbed detects level of Donkey Mouse IgG & has been published & validated for use in ELISA & WB.

EIA and Western blots: 1:5,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

물리적 형태

Immunoaffinity Purified and Immunodepletion

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA and 0.01% thimerosal.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.

저장 및 안정성

Maintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3