AC113

Anti-Neomycin Phosphotransferase II Antibody | AC113

from rabbit, purified by affinity chromatography

• 동의어(들): Aminoglycoside 3′-phosphotransferase, APH(3′)-II, APH(3′)II, Kanamycin kinase, type II, Neomycin-kanamycin phosphotransferase type II
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: polyclonal
• application: WB
• 종 반응성: human
• 기술: western blot: suitable
• citations: 5

속성

• 생물학적 소스: rabbit
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 기술: western blot: suitable
• NCBI 수납 번호: YP_788126
• UniProt 수납 번호: P00552
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin^®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

특이성

This antibody reacts to lysates from cells transfected or transformed with a plasmid containing a kanamycin/neomycin resistance gene, nptII (Aminoglycoside 3′-phosphotransferase).

면역원

Recombinant protein corresponding to full length neomycin phosphotransferase (NPT) II.

애플리케이션

Research Category
Secondary & Control Antibodies

Research Sub Category
Kinases & Phosphatases

품질

Evaluated by Western Blot in untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).

표적 설명

~29 kDa observed.

물리적 형태

Affinity purified

Hek 293 +/- transfection with pUSEamp(-)and neomycin treatment.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).

법적 정보

Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable