ABT58

Anti-phospho PKA-RII (Ser96) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): PRKAR2A, protein kinase, cAMP-dependent, regulatory, type II, alpha
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, rat
• 종 반응성(상동성에 의해 예측): mouse (based on 100% sequence homology)
• 기술: western blot: suitable
• NCBI 수납 번호: NP_032950
• UniProt 수납 번호: P12367
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer96)
• 유전자 정보: human ... PRKAR2A(5576)

설명

일반 설명

cAMP-dependent protein kinase type II-alpha regulatory subunit (PKA-RIIα) is an enzymatic protein belonging to the cAMP-dependent kinase regulatory chain family, a group of regulatory proteins involved in the PKA signaling pathway. This pathway has been shown to have regulatory roles in development, growth, and mortality of various organisms. PKA-RIIα is a subunit of the PKA type II regulatory chain, one of two PKA isoforms, and is involved in the regulation of membrane interactions through its binding to anchoring proteins, such as MAP2 kinase. Before its activation with cAMP, the enzyme is comprised of two catalytic chains and two regulatory chains, which split into two catalytic monomers and one regulatory dimmer after interacting with cAMP.

특이성

The antibody recognizes PKA-RIIalpha when phosphorylated at Ser96.

면역원

Epitope: Phosphorylated Ser96 of PKA-RIIalpha

KLH-conjugated linear peptide corresponding to mouse PKA-RIIalpha phosphorylated at Ser96.

애플리케이션

Research Category
Cell Structure

Signaling

Research Sub Category
Cytoskeletal Signaling

Kinases & Phosphatases

This Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).

품질

Evaluated by Western Blot in Forskolin untreated and treated PC12 cell lysates.

Western Blot Analysis: 1 µg/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 µg of Forskolin untreated and treated PC12 cell lysates.

표적 설명

~ 51 kDa MW observed. The specificity of the target was shown by Lambda phosphatase treatment.

결합

Replaces: 06-704

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Forskolin untreated and treated PC12 cell lysates

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable