ABT283

Anti-Cadherin-11/OB Cadherin Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Cadherin-11, OSF-4, Osteoblast cadherin, OB-cadherin
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, human
• 종 반응성(상동성에 의해 예측): rat (based on 100% sequence homology)
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NP_001788
• UniProt 수납 번호: P55287
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CDH11(1009)

설명

일반 설명

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.

면역원

Epitope: Cadherin 5 domain

KLH-conjugated linear peptide corresponding to the Cadherin 5 domain of Human Cadherin-11/OB Cadherin.

애플리케이션

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Cadherin-11/OB Cadherin Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Cadherin-11/OB Cadherin.

Western Blotting Analysis: 2 µg/mL of a representative lot of this antibody detected Cadherin-11/OB Cadherin in 10 µg of Mouse Brain, Mouse Brain Cytosol, Mouse Brain Membrane, Mouse Brain Nuclear Extract, Mouse (Aged) Brain Frontal Cortex, and Mouse Cerebellum tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution of a representative lot of this antibody detected Cadherin-11/OB Cadherin in neurons of the human cerebral cortex and thalamus, as well as purkinje and basket cells in human cerebellum.

품질

Evaluated by Western Blotting in Human Thalamus Tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Cadherin-11/OB Cadherin in 10 µg of Human Thalamus Tissue lysate.

표적 설명

~75 kDa observed.
Full length protein has a molecular weight of ~120 kDa, however the cleaved product has a molecular weight of ~75-80 kDa (McCusker, C., et al. (2009) MBC. 20(1):78-89). Uncharacterized bands may be observed in some lysates.

물리적 형태

Antigen Affinity Purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable