ABS574

Anti-Surfactant Protein D

from rabbit, purified by affinity chromatography

• 동의어(들): Pulmonary surfactant-associated protein D, Collectin-7, Lung surfactant protein D, PSP-D, SP-D
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 기술: immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_003010
• UniProt 수납 번호: P35247
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Pulmonary surfactant-associated protein D (UniProt P35247; also known as Collectin-7, Lung surfactant protein D, PSP-D, SP-D) is encoded by the SFTPD (also known as COLEC7, PSPD, SFTP4) gene (Gene ID 6441) in human. Surfactant, produced by alveolar type II cells and airway Clara cells, resides at the air-liquid interface of the lungs and is most commonly known for its role in reducing surface tension. Surfactant is a lipoprotein complex composed of approximately 10% proteins, including surfactant protein A (SP-A), SP-B, SP-C, and SP-D. SP-A and SP-D belong to the collectin family of proteins and can modulate innate immunity. SP-D is known to enhance pulmonary clearance of pathogens, such as Pseudomonous aerginosa, Klebsiella pneumonia, respiratory syncytial virus (RSV) and Influenza virus. SP-D is also shown to modify allergic responsee in the lungs by binding common allergens, including house dust mite and pollen particles. SP-A-deficient and SP-D-deficient mice are susceptible to bleomycin-induced lung injury and display increased cellular inflammation, more severe lung fibrosis, and reduced survival. In addition to lung, SP-D is also reported to be detected in extrapulmonary tissues, such as kidney and pancreas. Serum levels of either SP-A or SP-D in human are correlated with mortality in patients with pulmonary fibrosis. Human SP-D is produced with a signal peptide sequence (a.a. 1-20), which is removed posttranslationally to yield the mature protein (a.a. 21-375) that contains a collagen-like domain (a.a. 46-222), a coiled coil region (a.a. 223-252), and a C-type lectin domain (a.a. 260-375).

특이성

This rabbit polyclonal antibody targets a sequence within the C-type lectin domain of human surfactant protein D.

면역원

Epitope: domain of:

KLH-conjugated linear peptide corresponding to a sequence within the C-type lectin domain of human surfactant protein D.

애플리케이션

Detect Surfactant Protein D using this rabbit polyclonal Anti-Surfactant Protein D Antibody, Cat. No. ABS574, validated for use in Immunohistochemistry and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected surfactant protein D in human lung and pancreas tissue sections.

Research Category
Signaling

품질

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected surfactant protein D in 10 µg of A549 cell lysate.

표적 설명

~48 kDa observed. Target band size appears larger than the calculated molecular weights of 35.50/37.73 kDa (mature/pro-form) due to posttranslational modifications. Uncharacterized bands may be observed in some lysate(s).

결합

Replaces: AB3434

물리적 형태

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable