ABS45

Anti-phospho-MYPT1 (Thr696) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 분자량: (~130 kDa observed; 115.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).)
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): canine (based on 100% sequence homology), rat (based on 100% sequence homology), primate (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology), horse (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• 기술: inhibition assay: suitable (Peptide Inhibition Assay); western blot: suitable
• NCBI 수납 번호: NP_001137357.1
• UniProt 수납 번호: O14974
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pThr696)
• 유전자 정보: human ... PPP1R12A(4659)

설명

일반 설명

Anti-phospho-MYPT1 (Thr696), Cat. No. ABS45, is a rabbit polyclonal antibody that detects MYPT1 phosphorylated on Threonine 696 and is tested for use in Western Blotting and Peptide Inhibition Assay.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

특이성

This rabbit polyclonal antibody specifically detects MYPT1 phosphorylated on threonine 696.

면역원

Epitope: Phosphorylated Thr696

KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphothreonine 696 of human MYPT1.

애플리케이션

Anti-phospho-MYPT1 (Thr696) Antibody is an antibody against phospho-MYPT1 (Thr696) for use in WB.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Western Blotting (SNAP ID)Analysis: 0.1 µg/mL from a previous lot detected MYPT1 on 10 µg of NIH/3T3 cell lysate.

품질

Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody was used to detect MYPT1 phosphorylated at Thr696 on 10 µg of Calyculin/Okadaic treated and untreated HEK293 cell lysate.

표적 설명

Protein phosphatase 1 regulatory subunit 12A (UniProt: O14974; also known as Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit) is encoded by the PPP1R12A (also known as MBS, MYPT1) gene (Gene ID: 4659) in human. Myosin light chain phosphatase with its regulatory subunit, myosin phosphatase target subunit 1 (MYPT1), modulates Ca2+-dependent phosphorylation of myosin light chain by myosin light chain kinase, which is essential for smooth muscle contraction. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, which inhibits myosin phosphatase through the action of Rho-kinase. MYPT1 is localized on stress fibers and is distributed close to the cell membrane and at cell-cell contacts to regulate myosin phosphatase activity. It is phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696 that inhibits PP1C activity by an intramolecular mechanism. Mutations in PPP1R12A are involved in Genitourinary and/or brain malformation syndrome (GUBS), an autosomal dominant syndrome that is characterized by multiple congenital anomalies including urogenital malformations and brain abnormalities. Five isoforms of MYPT1 have been described that are produced by alternative splicing. (Ref.: Qiao, Y-N., et al. (2014). J. Biol. Chem. 289(32); 22512-22523).

결합

Replaces: 07-251

물리적 형태

Affinity purified

Purified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Calyculin/Okadaic treated and untreated HEK293 cell lysates

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable