ABS2130M

Anti-phospho RasGRP2 (Ser116/Ser117)

from rabbit

• 동의어(들): RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, F25B3.3 kinase-like protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: mouse, monkey
• 종 반응성(상동성에 의해 예측): rat (based on 100% sequence homology)
• 포장: antibody small pack of 25 μg
• 기술: immunofluorescence: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_035372
• UniProt 수납 번호: Q9QUG9
• 타겟 번역 후 변형: phosphorylation (pSer116/pSer117)
• 유전자 정보: mouse ... Rasgrp2(19395)

설명

일반 설명

RAS guanyl-releasing protein 2 (UniProt: Q9QUG9; also known as Calcium and DAG-regulated guanine nucleotide exchange factor I, RasGRP2, CalDAG-GEFI, F25B3.3 kinase-like protein) is encoded by the Rasgrp2 gene (Gene ID: 19395) in murine species. RasGRP proteins are considered as activators of Ras and other related small GTPases by virtue o functioning as guanine nucleotide exchange factors (GEFs). RasGRP-1 through -4 share many structural domains, however, they display subtle differences between each of the different family members. They are expressed in distinct patterns, such as in different cells of the hematopoietic system and in the brain. RasGRP2′s expression is restricted to platelets and their precursors, megakaryocytes as well as neutrophils. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS, but not HRAS. It is also reported to functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils, probably through inside-out integrin activation. RaGRP2 is found both in the cytosol and associated with membranes. It is shown to be enriched at juxtamembrane areas and membrane ruffles. Three isoforms of RasGRP2 have been reported that are generated by alternative splicing. It contains two EF-hand domains, one N-terminal Ras-GEF domain, one phorbol-ester/DAG-type zinc finger domain, and one Ras-GEF domain. RasGRP2 deficient animals exhibit bleeding problems due to defects in platelets aggregation and degranulation. (Ref.: Olga Ksionda, O et al. (2013). Front Biol (Beijing). 8(5): 508 532).

특이성

This rabbit polyclonal antibody detects RAS guanyl-releasing protein 2 phosphorylated on Ser116/117 in Mouse and Monkey cells. It targets an epitope with in 12 amino acids surrounding Ser116/Ser117 from the N-terminal region.

면역원

A linear peptide corresponding to 12 amino acids surrounding phosphorylated Ser116/117 from the N-terminal region of murine RAS guanyl-releasing protein 2.

Epitope: unknown

애플리케이션

Anti-phospho RasGRP2 (Ser116/Ser117), Cat. No. ABS2130, is a rabbit polyclonal antibody that detects RAS guanyl-releasing protein 2 phosphorylated on Ser116/Ser117 and has been tested for use in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A representative lot detected phospho RasGRP2 (Ser116/Ser117) in nucleus accumbens (NAc) from Drd1-mVenus transgenic mice treated with cocaine (Nagai, T., et. al. (2016). Neuron. 89(3):550-65).

Western Blotting Analysis: A representative lot detected phospho RasGRP2 (Ser116/Ser117) in RasGRP2 purified from GST-Rasgrp2 transfected COS-7 cells and phosphorylated by GST-PKAc alpha, Forskolin stimulated mouse striatal muscle slices (Courtesy of Dr. Kozo Kaibuchi at Nagoya University, Japan).

Western Blotting Analysis: A representative lot detected phospho RasGRP2 (Ser116/Ser117) in a kinase assay with GST-Rasgrp2 (amino acids 1-374) and PKA, Forskolin and SKF81279 stimulated mouse striatal muscle slices, COS-7 cells transfected with EGFP-Rasgrp2 and Myc-PKA, nucleus accumbens (NAc) from mice treated with cocaine, Nucleus accu (Nagai, T., et. al. (2016). Neuron. 89(3):550-65).

Research Category
Signaling

품질

Evaluated by Western Blotting in RAS guanyl-releasing protein 2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser116/Ser117 by GST-PKAc alpha.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RAS guanyl-releasing protein 2 in RasGRP2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser116/Ser117 by GST-PKAc alpha

표적 설명

~90 kDa observed; 69.45 kda calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 10% Ethylene Glycol, pH 7.6 with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.