ABS1526

Anti-phospho-AKT1 Antibody, Thr479

from rabbit, purified by affinity chromatography

• 동의어(들): AKT alpha, Protein kinase B, RAC-alpha serine/threonine-protein kinase, Protein kinase B alpha, PKB, Proto-oncogene c-Akt, RAC-PK-alpha
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: rat
• 종 반응성(상동성에 의해 예측): human (based on 100% sequence homology), mouse (based on 100% sequence homology)
• 포장: antibody small pack of 25 μg
• 기술: inhibition assay: suitable (peptide); western blot: suitable
• NCBI 수납 번호: NP_001014431.1
• UniProt 수납 번호: P31749
• 배송 상태: ambient
• 타겟 번역 후 변형: phosphorylation (pThr479)
• 유전자 정보: human ... AKT1(207)

설명

일반 설명

RAC-alpha serine/threonine-protein kinase (UniProt: P31749; EC: 2.7.11.1; Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1) is encoded by the AKT1 (also known as PKB, RAC) gene (Gene ID: 207) in human. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) that regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. In mammals these 3 isoforms of AKT exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. AKT1 is constitutively phosphorylated on Ser124, in the region between PH and catalytic domains, and on Thr450, in the C-terminal region in unstimulated cells. Phosphorylation at Thr308 and Ser473 induces complete activation of AKT1. AKT1 contains one PH domain (aa 5-108), one protein kinase domain (aa 150-408), and one AGC-kinase C-terminal domain (aa 409-480). Binding of the PH domain to phosphatidylinositol 3,4,5-trisphosphate (PIP3) results in its targeting to the plasma membrane. AKT1 can be phosphorylated at Ser477/Thr479 by Cdk2/Cyclin A, which locks it into an active conformation. Ser477/Thr479 phosphorylation is believe to trigger Akt1 activation either through enhancing the association between AKT1 and mTORC2 to promote phosphorylation of Ser473, or by functionally compensating for phosphorylated Ser473 to lock AKT1 in its active conformation. AKT1 phosphorylated at Ser477/Thr479 is reported to display increased association with Sin1 and mTOR complexes. (Ref.: Liu, P et al (2014). Nature. 508(7497): 541-545).

특이성

This rabbit polyclonal antibody detects AKT1 phosphorylated on Thr479.

면역원

KLH-conjugated linear peptide corresponding to 10 amino acids surrounding Thr479 from the C-terminal region of human AKT1.

애플리케이션

Anti-phospho-AKT1, Thr479, Cat. No. ABS1526, is a rabbit polyclonal antibody that detects AKT1 phosphorylated on Thr479 and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Peptide Inhibition Analysis: A 1:500 dilution from a representative lot detected phospho-AKT1, Thr479 in RAT2 cells.

Research Category
Signaling

품질

Evaluated by Western Blotting in RAT2 cells transfected with Src (21-154).

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-AKT1, Thr479 in lysate of RAT2 cells transfected with Src (21-154).

표적 설명

~56 kDa observed; 55.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable