ABN905

Anti-alpha-Synuclein

from rabbit, purified by affinity chromatography

• 동의어(들): Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, mouse, rat
• 포장: antibody small pack of 25 μg
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NM_000345.3
• UniProt 수납 번호: P37840
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SNCA(6622)

설명

일반 설명

Alpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is abundantly expressed in the brain and is found in a classic amyloid fibril form within the intra-neuronal Lewy body deposits of Parkinson′s disease brains. The physiological function of synucleins is not well understood, but appears to involve membrane interactions, and in particular reversible binding to synaptic vesicle membranes. It may also be involved in the regulation of dopamine release and transport. It is also reported to reduce neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Genetic alterations of SNCA gene is reported to result in aberrant polymerization into fibrils, which is associated with several neurodegenerative diseases (synucleinopathies). Mutations in SNCA gene are also known to Parkinson′s disease characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology of Parkinson s disease involves progressive loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies in surviving neurons in various areas of the brain.

특이성

This rabbit polyclonal antibody detects alpha synuclein in human, mouse, and rat. It targets an epitope with in 21 amino acids from the C-terminal region.

면역원

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Alpha-Synuclein.

애플리케이션

Anti-alpha-Synuclein, Cat. No. ABN905, is a rabbit polyclonal antibodt that detects alpha synuclein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: A 1:1,000-2,000 dilution from a representative lot detected alpha-Synuclein in human and rat brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:2,000-3,000 dilution from a representative lot detected alpha-Synuclein in human cerebral cortex and rat cerebellum tissue sections.

품질

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected alpha-Synuclein in mouse brain tissue lysate.

표적 설명

~17 kDa observed; 14.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

기타 정보

Concentration: Please refer to lot specific datasheet.