ABN897

Anti-AXL (UFO) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Tyrosine-protein kinase receptor UFO, AXL oncogene
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_068713
• UniProt 수납 번호: P30530
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... AXL(558)

설명

일반 설명

Tyrosine-protein kinase receptor UFO (EC 2.7.10.1; UniProt P30530; also known as AXL oncogene) is encoded by the AXL (also known as UFO) gene (Gene ID 558) in human. AXL/UFO, MERTK, and TYRO3 constitute the TAM family single transmembrane receptor tyrosine kinases (RTKs) similar overall domain structure, including two extracellular C2-type Ig-like domains (human AXL a.a. 27-128 & 139-222) and two type-III fibronectin domains (human AXL a.a. 227-331 & 336-428), as well as a conserved intracellular kinase domain (human AXL a.a. 536-807) with a unique KWIAIES sequence (human AXL a.a. 714-720). The activation of TAM RTKs involves lipid phosphatidylserine (PtdSer) and γ-carboxylated proteins that interact simultaneously with TAM RTKs N-terminal region (human AXL a.a. 26-92) via their C-terminal domain and PtdSer with their N-terminus, effectively acting as "bridging ligands". Growth arrest-specific protein 6 (GAS6) and vitamin K-dependent protein S (PROS1) are the first two characterized ligands, with GAS6 targeting all three TAM RTKs and PROS1 binding only to MERTK and TYRO3. PtdSer is generally made available for TAM RTKs activation when externalized on apoptotic cell membranes, aggregating platelets, exosomes and invading virus envelopes. Additional tissue- and receptor-specific TAM RTK ligands have been identified. Unlike MERTK and TYRO3, AXL homodimerization and near-complete activation can be achieved via a 2:2 stoichiometric interaction with GAS6 without PtdSer-presenting membranes. TAM RTKs collaboratively stabilize clot formation upon their activation by PtdSer on the surface of aggregating platelets. TAM RTKs are aberrantly expressed and activated in virtually every type of cancer, where their functions in terminating innate immune-mediated inflammation and natural killer (NK) cell responses help promote cancer survival.

특이성

This rabbit polyclonal antibody targets cytoplasmic C-terminal end region present in both spliced isoforms of human AXL (UFO) reported by UniProt (P30530). This antibody will not detect internalized portion, but not the extracellular portion of the receptor following its cleavage by ADAM10.

면역원

Epitope: C-terminus

GST-tagged human AXL (UFO) C-terminal end fragment lacking most of the kinase domain sequence.

애플리케이션

Anti-AXL (UFO), Cat. No. ABN897, is a highly specific rabbit polyclonal antibody that targets receptor tyrosine kinase AXL (UFO) and has been tested in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected AXL (UFO) in HeLa cells.

Research Category
Neuroscience

품질

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AXL (UFO) in 10 µg of HeLa cell lysate.

표적 설명

~140 kDa observed. Target band size appears larger than the calculated molecular weights of 98.34/95.47 kDa (pro-/mature isoform Long) and 97.38/94.51 kDa (pro-/mature isoform Short) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable