ABN2252

Anti-Neuroligin-4

• 동의어(들): Neuroligin Y
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 종 반응성: human
• 포장: antibody small pack of 100
• 기술: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG
• 에피토프 서열: C-terminal cytoplasmic domain
• 단백질 ID 수납 번호: NP_055708
• UniProt 수납 번호: Q8NFZ3
• 유전자 정보: human ... NLGN4Y(22829)

설명

특이성

This rabbit polyclonal antibody detects Neuroligin-4. It targets an epitope within 16 amino acids from the C-terminal, cytoplasmic domain.

면역원

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal, cytoplasmic domain of human Neuroligin-4.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cells overexpressing Human Neuroligin-4.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neuroligin-4 in lysate from HEK293T cells overexpressing Human Neuroligin-4.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Neuroligin-4 in Immunofluorescence applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Western Blotting Analysis: A representative lot detected Neuroligin-4 in Western Blotting applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Neuroligin-4 in Immunoprecipitation applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Neuroligin-4 (UniProt: Q8NFZ3; also known as Neuroligin Y) is encoded by the NLGN4Y (also known as KIAA0951) gene (Gene ID: 22829) in human. Neuroligins are postsynaptic cell adhesion molecules that have crucial roles in synapse maturation and function. In humans, NLGN4X is located on the X chromosome and the NLGN4Y is localized on the Y chromosome and together they form an X-Y pair. They share about 97% amino acid identity; however, they are differentially regulated. Although several mutations in NLGN4X have been linked to autism, only one mutation has been identified in NLGN4Y gene. Despite their high degree of sequence identity, NLGN4Y exhibits severe deficit in trafficking to cell surface and related synaptic effects. This has been attributed to serine 93 in NLGN4Y. A substitution of serine to proline at residue 93 is shown to rescue it and increase its surface trafficking. Neuroligin-4 is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-43), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 44-676), a transmembrane domain (aa 677-697), and a cytoplasmic domain (aa 698-816). Its expression is observed in fetal and adult brain, prostate, and testis. It serves as a neuronal cell surface protein that is involved in cell-cell interactions. (Ref.: Nguyen, TA., et al. (2020). Neuron 106(5); 759-768; Marro, SG., et al. (2019). Neuron. 103(4); 617-626).

물리적 형태

Purified rabbit polyclonal antibody in buffer containing PBS, pH 7.4, without azide.

재구성

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable