ABN194

Anti-EVI-5 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Ecotropic viral integration site 5 protein homolog, EVI-5
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, rat
• 종 반응성(상동성에 의해 예측): human (immunogen homology)
• 기술: immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_005656
• UniProt 수납 번호: O60447
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EVI5(7813)

설명

일반 설명

Also known as Ecotropic viral integration site 5 protein homolog, EVI-5 serves as a key regulator of the cell cycle through time-dependent stabilization of Emi1, FBX05 protein, and APC/C ubiquitination substrates, such as cyclin A. EVI-5 accumulates in early G1 following Plk1 destruction, but is degraded in a Plk1- and ubiquitin-dependent manner in early mitosis. EVI-5 ablation causes premature APC/C activation, SCF(betaTrCP)-dependent Emi1 degradation, and cyclin destruction, all of which leads to cell cycle arrest and cytokinesis failure. It exists as a monomer and a dimer, and it may form complexes with alpha- and gamma-tubulin or interact with the INCENP-aurora B kinase-survivin chromosomal passenger complex. EVI-5 is implicated in abnormal states, such as multiple sclerosis and neuroblastoma.

면역원

KLH-conjugated linear peptide corresponding to the human EVI-5.

애플리케이션

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This EVI-5 antibody is validated for use in IHC & WB for the detection of the EVI-5 protein.

Western Blot Analysis: 2 µg/mL from a representative lot detected EVI-5 in NIH/3T3 nuclear extract.

품질

Evaluated by Immunohistochemistry in mouse and rat brain tissues.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected EVI-5 in mouse and rat brain tissues.

표적 설명

~95 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~17 kDa, and ~15 kDa in some cell lysates. This protein is known to have three potential phosphorylation sites.

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Mouse and rat brain tissues

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable