ABN1714

Anti-Rootletin

from rabbit, purified by affinity chromatography

• 동의어(들): Ciliary rootlet coiled-coil protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: polyclonal
• application: ICC; IHC; IP
• 종 반응성: mouse
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable
• citations: 2

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable
• NCBI 수납 번호: NP_742120.2
• UniProt 수납 번호: Q8CJ40
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Crocc(230872)

설명

일반 설명

Rootletin (UniProt: Q8CJ40; also known as Ciliary rootlet coiled-coil protein) is encoded by the Crocc (also known as Kiaa0445) gene (Gene ID: 230872) in murine species. Rootletin is a major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells that originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. It contributes to centrosome cohesion before mitosis. In ciliated cells, it is associated with ciliary rootlets, but in non-ciliated cells it is localized between, around and at the proximal ends of the centrioles. It dissociates from the centrioles at the onset of D7mitosis and re-associates with them at anaphase. Highest expression of Rootletin is found in photoreceptor cells of retina. Lower expressions have been reported in brain, trachea and kidney. In retinal photoreceptors rootlets extend from the basal bodies to the synaptic terminals and anchor endoplasmic reticulum membranes along their length. During embryonic development, it is enriched along the apical domains of neuro epithelium in brain ventricular zone, in primordia of retinal pigment epithelia, and in neural retina. Rootletin contains a globular head domain and a tail domain that consists of extended coiled-coil structures. The head domain may be required for targeting to the basal body and binding to a kinesin light chain. (Ref.: Yang, J., et al. (2002). J. Cell Biol. 159(3): 431-440).

특이성

This rabbit polyclonal antibody detects Rootletin in murine hippocampus and cerebral cortex. It targets an epitope within 188 amino acids from the C-terminal region.

면역원

His-tagged recombinant fragment corresponding to 188 amino acids from the C-terminal region of murine Rootletin.

애플리케이션

Anti-Rootletin Antibody, Cat. No. ABN1714, is a highly specific rabbit polyclonal antibody that targets Rootletin and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Immunoprecipitation.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Rootletin in mouse cerebral cortex and mouse retina tissues.

Immunocytochemistry Analysis: A representative lot detected Rootletin in Immunocytochemistry applications (Veleri, S., et. al. (2014). Nat Commun. 5:4207).

Immunoprecipitation Analysis: A representative lot detected Rootletin in Immunoprecipitation applications (Yang, J., et. al. (2002). J Cell Biol. 159(3):431-40).

Immunofluorescence Analysis: A representative lot detected Rootletin in Immunofluorescence applications (Sun, X., et. al. (2012). Cilia. 1(1):21).

Research Category
Neuroscience

품질

Evaluated by Immunohistochemistry in mouse hippocampus.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Rootletin in mouse hippocampus.

표적 설명

~227 kDa observed; 226.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Purified rabbit polyclonal antibody in PBS with 50% glycerol and without azide.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2