ABN1459

Anti-Nkx6.1

from rabbit, purified by affinity chromatography

• 동의어(들): Homeobox protein Nkx-6.1, Homeobox protein NK-6 homolog A
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, mouse
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_006159.2
• UniProt 수납 번호: P78426
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NKX6-1(4825)

설명

일반 설명

Homeobox protein Nkx-6.1 (UniProt: P78426; also known as Homeobox protein NK-6 homolog A) is encoded by the NKX6-1 (also known as NKX6A) gene (gene ID: 4825) in human. Nkx-6.1 is a transcription factor that binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. It is expressed in pancreatic beta cells and is involved in transcriptional regulation in islet beta cells. During pancreatic development both Pdx-1 and Nkx-6.1 are observed in rat pancreas. However, later Nkx-6.1 became more beta-cell-restricted, and Pdx-1 is found in other islet cell types and in the duodenal epithelium. Nkx-6.1 binds to the insulin promoter and is involved in regulation of the insulin gene. The C-terminal domain of Nkx-6.1 contributes to sequence-specific DNA-binding. Together with NKX2-2 and IRX3 it acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by Sonic hedgehog (Shh) signals. Although Shh signaling is required for Nkx-6.1 expression in the ventral neural tube and spinal meninges, a Shh-independent pathway appears to operate in regulating Nkx-6.1 expression in the foregut region. (Ref.: Cai, J., et al. (2000). Genesis. 27(1); 6-11; Oster, A., et al. (1998). J. Histochem. Cytochem. 46(6); 707-715).

특이성

This rabbit polyclonal antibody detects Homeobox protein Nkx-6.1 in human cells. It targets an epitope within 61 amino acids from the N-terminal region.

면역원

GST/His-tagged recombinant fragment corresponding to 61 amino acids from the N-terminal region of human Homeobox protein Nkx-6.1.

애플리케이션

Anti-Nkx6.1, Cat. No. ABN1459, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Nkx6.1 in human pancreas and mouse pancreas tissue sections.

Research Category
Neuroscience

품질

Evaluated by Western Blotting in PANC-1 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Nkx6.1 in PANC-1 cell lysate.

표적 설명

~54 kDa observed; 37.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1