ABN1379

Anti-TAG-1 Antibody

serum, from rabbit

• 동의어(들): Contactin-2, TAG-1, Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: rat, mouse
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_037016
• UniProt 수납 번호: P22063
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Cntn2(21367) ; rat ... Cntn2(25356)

설명

일반 설명

Contactin-2 (UniProt P22063; also known as Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1) is encoded by the Cntn2 (also known as Tax1) gene (Gene ID 25356) in rat species. Proper localization of the voltage-gated Na+ channels to the node of Ranvier and voltage-gated K+ channels to the juxtaparanode (JXP) along myelinated axons is essential for the fast propagation of action potentials. Localization of clustered ion channels in specific membrane domains depends on specific cell adhesion molecules (CAMs), as well as on the integrity of neighboring domains. Contactin-2/TAG-1 and Caspr2 form a membrane scaffold complex that functions to cluster Kv1 potassium channels at the JXP. Deletion of either of these CAMs causes Kv1 channels to disappear from the JXP and to redistribute along the internode. Contactin-2/TAG-1 is initially produced with an N-terminal signal peptide (a.a. 1-30) and a C-terminal propeptide (a.a. 1016-1040) sequence, the removal of which yields the mature GPI-anchored membrane glycoprotein.

면역원

Epitope: Fibronectin type-III domains 1-4.

Fc-tagged recombinant protein corresponding to the Fibronectin type-III domains 1-4 of rat TAG-1.

애플리케이션

Anti-TAG-1 Antibody is an antibody against TAG-1 for use in Western Blotting, Immunocytochemistry.

Immunocytochemistry Analysis: Representative lots detected TAG-1 immunoreactivity at the juxtaparanodal (JXP) region of teased sciatic nerves from wild-type, but not Caspr−/−, Caspr2−/−, or Caspr−/−Caspr2−/− double knockout, mice. Reexpressing full-length Caspr2, but not 4.1-binding sequence (d4.1) deletion Caspr2 mutant, in Caspr2−/− transgenic mice restored JXP TAG-1 immunoreactivity (Gordon, A., et al. (2014). J. Neurosci. 34(45):14820 –14826; Horresh, I., et al. (2010). J. Neurosci. 30(7):2480–2489).
Immunocytochemistry Analysis: A representative lot detected a loss of TAG-1 immunoreactivity at the juxtaparanodal (JXP) region of teased sciatic nerves isolated from 4.1B−/− mice lacking the cytoskeletal adapter protein 4.1B (Horresh, I., et al. (2010). J. Neurosci. 30(7):2480–2489).

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

품질

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected TAG-1 in 10 µg of mouse brain tissue lysate.

표적 설명

~140 kDa observed. Target band appears larger than the calculated molecular weight of 107.3 kDa due to glycosylation.

물리적 형태

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable