추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
rat, human
종 반응성(상동성에 의해 예측)
mouse (based on 100% sequence homology), primate (based on 100% sequence homology), equine (based on 100% sequence homology), bovine (based on 100% sequence homology), feline (based on 100% sequence homology)
기술
immunoprecipitation (IP): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ITSN1(6453)
일반 설명
Intersectin-1 (UniProt Q15811; also known as Human intersectin-SH3 domain-containing protein SH3P17, SH3 domain-containing protein 1A, SH3P17, Src homology 3 domain-containing protein 1A) is encoded by the ITSN1 (also known as ITSN, SH3D1A) gene (Gene ID 6453) in human. Intersectin-1 and the related intersectin-2 are molecular scaffolds that play essential roles at the early stages of clathrin-mediated endocytosis (CME) by clustering the membrane-sculpting FCHo1/2 proteins that mark the assembly sites of clathrin-coated pits. Both ITSNs are widely expressed in tissues and share the same domain organization. Both ITSN transcripts are subjected to alternative splicing, generating short and long isoforms. The short splice form (ITSN-s) comprises two Eps15 homology (EH) domains, a coiled-coil region (CCR) and five Src 3 homology (SH3) domains. The long splice variant (ITSN-L) contains an additional Dbl homology (DH) GDP/GTP exchange domain, a plekstrin homology (PH) and C2 domains. Most of these domains mediate protein-protein interactions necessary for the assembly of multi-protein complexes. In addition to CME, ITSN1 is known to interact with multiple proteins involved in exocytosis, mitogenic signaling, actin cytoskeleton remodeling and apoptosis. ITSN1 mRNA is reported to be elevated in patients with Down syndrome and Alzheimer’s disease, and ITSN1 over-expression increases aggregation of mutant huntingtin in cultured cells.
특이성
This antiserum detects ITSN1, but not ITSN2. This antiserum targets EH domain sequence common to all 13 spliced variants of ITSN1 (UniProt Q15811-1 through Q15811-13). The long isoform ITSN-l (UniProt Q15811-1) is neuron-enriched and can be detected at a high level in brain. ITSN-l (~200kDa) could also be detected at low levels in some, but not all non-neuronal tissues and cell lines.
면역원
Epitope: EH2 domain
His-tagged recombinant protein corresponding to the EH2 domain of human Intersectin-1/ITSN1.
애플리케이션
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Ruk/CIN85 with transiently expressed Omni-tagged ITSN1 in an EGF stimulation-independent manner using a stable MCF-7 culture overexpressing Ruk/CIN85 (Novokhatska, O., et al. (2009). Cell Signal. 21(5):753-759).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated ITSN2 with ITSN1 from HEK293 cells (Novokhatska, O., et al. (2013). PLoS One. 8(7):e70546).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ITSN1 in lysates prepared from growing HEK293, HeLa, MCF-7, and MDA-MB-231 cultures (Novokhatska, O., et al. (2013). PLoS One. 8(7):e70546).
Western Blotting Analysis: A representative lot detected ITSN1, but not ITSN2, by Western blotting using total HEK293 cell lysate or ITSN1 immunoprecipitate obtained from HeLa cell lysates (Novokhatska, O., et al. (2013). PLoS One. 8(7):e70546).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated ITSN2 with ITSN1 from HEK293 cells (Novokhatska, O., et al. (2013). PLoS One. 8(7):e70546).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ITSN1 in lysates prepared from growing HEK293, HeLa, MCF-7, and MDA-MB-231 cultures (Novokhatska, O., et al. (2013). PLoS One. 8(7):e70546).
Western Blotting Analysis: A representative lot detected ITSN1, but not ITSN2, by Western blotting using total HEK293 cell lysate or ITSN1 immunoprecipitate obtained from HeLa cell lysates (Novokhatska, O., et al. (2013). PLoS One. 8(7):e70546).
This Anti-Intersectin-1/ITSN1 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Intersectin-1/ITSN1.
품질
Evaluated by Western Blotting in rat brain cytosol tissue lysate.
Western Blotting Analysis: Western Blotting Analysis: 0.5 µg/mL of this antibody detected Intersectin-1/ITSN1 in 10 µg of rat brain cytosolic preparation.
Western Blotting Analysis: Western Blotting Analysis: 0.5 µg/mL of this antibody detected Intersectin-1/ITSN1 in 10 µg of rat brain cytosolic preparation.
표적 설명
~160 kDa observed. Target band size(s) may vary depending on the isoform(s) present in the sample(s).
물리적 형태
Format: Purified
기타 정보
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Oleksii Nikolaienko et al.
Cellular signalling, 21(5), 753-759 (2009-01-27)
Intersectin 1 (ITSN1) is an adaptor protein involved in clathrin-mediated endocytosis, apoptosis, signal transduction and cytoskeleton organization. Here, we show that ITSN1 forms a complex with adaptor protein Ruk/CIN85, implicated in downregulation of receptor tyrosine kinases. The interaction is mediated
Olga Novokhatska et al.
PloS one, 8(7), e70546-e70546 (2013-08-13)
Scaffolding proteins of the intersectin (ITSN) family, ITSN1 and ITSN2, are crucial for the initiation stage of clathrin-mediated endocytosis. These proteins are closely related but have implications in distinct pathologies. To determine how these proteins could be separated in certain
Claudia Puri et al.
Developmental cell, 53(2), 154-168 (2020-04-22)
Autophagy involves engulfment of cytoplasmic contents by double-membraned autophagosomes, which ultimately fuse with lysosomes to enable degradation of their substrates. We recently proposed that the tubular-vesicular recycling endosome membranes were a core platform on which the critical early events of
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